Effectiveness of Fiscal policy ?

Hi all I am about to start a research / Dissertation in the area of fiscal policy in Australia. More specifically I Would like to start off with a Dissertation at Masters level leading to a...

04 November 2019 7,491 3 View

How to interpret the effect of wear after Pin on disc test using SEM?

what is the purpose of taking SEM image after Pin on disc test,if so how to identify the wear mechanism.

09 May 2018 4,630 4 View

'DIFFERENCE' not defined in SetByBoolean

17 July 2017 559 3 View

RNA degradation problem, no 18s/28s peaks?

I am trying to extract RNA from primary culture using Trizol. But after checking on the bioanalyzer I obtained very low RIN value (3.4) and the electropherogram showed no peaks (only the marker...

10 April 2017 2,777 3 View

Anyone can tell me how to look for minisatellites in genome of an organism?

anyone can tell me how to look for minisatellites in genome of an organism.

11 January 2017 630 4 View

How to formulate phytoconstituent terpenoid in drug dosage form?

Hi All My plant of interest contain terpenoids,I request your kind suggestions in formulating terpenoids as dosage form and any possibility in formulation in nanoscience

01 September 2016 1,802 1 View

Can someone please explain how does polysome profiling data reveal the active translational state of mRNas?

I am basically interested in understanding the graph obtained after ribosome profiling and how can you know from the graph in which translational state are the mRNAs?

01 August 2016 4,482 3 View

Synthesis /Semisynthesis of natural product ? Design of synthesis procedure for natural product

Hi All I want to test my isolated phytoconstituent for some biological activity( i.e antidiabetic or anticancer).How to screen individual phytoconstituent or the synthetic analog is potent?. How...

26 April 2016 9,936 3 View

Can we study the anticancer activity of two phytoconstituents (in combination) to treat inflammation/cancer in cell lines/animal models ?

Hi All I am planning to study the molecular mechanism that link inflammation and cancer. I request you to advice me that whether two phytoconstituents(which share same biological property) can be...

25 March 2016 3,313 3 View

Clone having repetitive sequence insert and its discripancy upon scale up?

I have cloned my 3.3Kb insert having repetitive sequence in yeast expression vector, by directional cloning in E.coli. Positive Clone of 8.7Kb was confirmed by restriction analysis. While the same...

26 January 2016 4,477 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View