Zhangrui Tang

7 Questions 3 Answers 0 Followers

Questions related from Zhangrui Tang

Why does leaky protein expression occur?

I have a question about leaky protein expression. Our lab has stored GFP plasmids. Previously, when we transformed this plasmid into E. coli, there was no leaky expression. However, after a recent...

09 June 2025 6,977 2 View

Does anyone have a map of the pGro12 plasmid?

After extensive research, I found information only on the pGro7 plasmid. However, the inducing agents for pGro7 and pGro12 differ. I aim to use IPTG for protein expression, and literature...

03 May 2025 8,680 1 View

When there are numerous repetitive sequences in my gene sequence, how can I design primers for PCR?

I have a gene in hand. If this gene is divided into four domains, namely A, B, A, and B, the sequences of the two A domains and the two B domains are identical, but the gene of the first B domain...

25 November 2024 3,476 2 View

There is a protein band before and after induction of E. coli, but after sonication, there is no protein in the pellet and supernatant. Why?

05 October 2023 1,996 3 View

Does anyone know the reason for the uneven baseline of size exclusion chromatography ?

I installed a superdex 75 pg myself, and the conductance baseline was flat at the beginning of the balance, and then the baseline was flat when the sample was eluted to 50%, and the last 50%...

07 July 2023 8,627 3 View

Is the salt concentration eluted from the ion exchange column the lowest salt concentration that the protein tolerates?

I have a protein, when the salt concentration slowly drops to 300mM, there will be some precipitation, and the salt concentration eluted on the ion exchange column is also 300mM.

29 June 2023 4,038 0 View

Do Competent Cells Affect Molecular Cloning?

I made some competent cells, but using those cells for molecular cloning will produce a lot of wrong plasmids, and the colonies grow very few, so will competent cells affect molecular cloning to...

01 January 1970 8,384 1 View