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Questions related from Zhangrui Tang
I have a question about leaky protein expression. Our lab has stored GFP plasmids. Previously, when we transformed this plasmid into E. coli, there was no leaky expression. However, after a recent...
09 June 2025 6,969 2 View
After extensive research, I found information only on the pGro7 plasmid. However, the inducing agents for pGro7 and pGro12 differ. I aim to use IPTG for protein expression, and literature...
03 May 2025 8,673 1 View
I have a gene in hand. If this gene is divided into four domains, namely A, B, A, and B, the sequences of the two A domains and the two B domains are identical, but the gene of the first B domain...
25 November 2024 3,471 2 View
There is a protein band before and after induction of E. coli, but after sonication, there is no protein in the pellet and supernatant. Why?
05 October 2023 1,991 3 View
I installed a superdex 75 pg myself, and the conductance baseline was flat at the beginning of the balance, and then the baseline was flat when the sample was eluted to 50%, and the last 50%...
07 July 2023 8,620 3 View
I have a protein, when the salt concentration slowly drops to 300mM, there will be some precipitation, and the salt concentration eluted on the ion exchange column is also 300mM.
29 June 2023 4,033 0 View
I made some competent cells, but using those cells for molecular cloning will produce a lot of wrong plasmids, and the colonies grow very few, so will competent cells affect molecular cloning to...
01 January 1970 8,379 1 View