What is the role of ether solvent (such as THF) in Sonogashira coupling?

Can Amine (such as Et3N) be used as solvent as well as base for the Sonogashira Coupling? How to decide the amount of solvent to be used in any reaction? Does the amount of solvent affect the...

14 February 2021 4,018 3 View

What is the posiblities of climate change in developing countries and in Nepal particular ? Typically in trans-Himalayan regions ?

positives of climate change, future options of food security, future options of land expansion for new farmland.

07 December 2020 4,220 6 View

How Architecture is related with Culture?

How architectural form, shape, style and technique are influenced by Cultural Values and Norms?

05 October 2020 6,651 17 View

Do we really pay serious attention to balance such factors for good design affecting in Human comfort?

It has been recognized that Architecture is influenced by many factors like, Social, Physical, Cultural, Environmental, Economic and Political etc.

04 October 2020 5,867 10 View

No title

Dear All, I'm looking for a freely available software /tool/script for the detection and location of local earthquakes using data from my own network of more than 20 vertical component...

29 September 2020 6,178 8 View

RNA extraction from White Calla lily leaf using Trizol method?

I am trying to extract RNA from leaf of White Calla lily by using Trizol method. By using this method, i am getting good concentration value and 260/280 ratio on nano drop measurement as well,...

06 September 2020 1,780 3 View

What are the best possible research Questions related to COVID-19 for social scientists ?

We are now dealing with a COVID world and a post-COVID world lies in our future. Good social research will generate better understandings not only of the current social impact of COVID, but also...

16 April 2020 6,999 16 View

Which circuit design should I use to generate emf oscillating at frequency of 2.5Ghz ?

I am looking for a circuit which can generate an emf oscillating at frequency of 2.5Ghz. My setup is in such a way that, I am passing particles (negligible mass) from a tunnel and want an emf...

21 December 2019 9,800 6 View

Why Biosurfactant production varies with the carbon sources?

Some Pseudomonas sp. are reported for biosurfactant production with glucose as a carbon source, where as other Pseudomonas sp. reports the significant BS production with hydrocarbon. Why?

12 November 2019 282 3 View

What is the role of nitrobenzene in the synthesis of 1,3,6,8-tetrabromopyrene ?

During the synthesis of 1,3,6,8-tetrabromopyrene, nitrobenzene was used as solvent, so I feel that it was used as a solvent may be because the reaction requires high temperature to form the...

03 July 2019 2,534 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Does sds interfere with immunization?

I want to extract a protein from polyacrylamide gel to be used for immunization. I am wondering if I need to run PAGE since sds might interfere with the immunization process. Thank you

10 February 2021 8,568 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View