How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Can there be a decrease in the membrane time constant and an increase in membrane excitability?

In my data set, I am seeing exposure to my peptide of interest leads to significant increases in action potential firing responses. But in the same cell types, I am seeing a decrease in the...

17 November 2020 5,441 4 View

What is the best anatomic plane to study BNST neurons using ex vivo slice culture in a rat?

I am looking to study neurons of the bed nucleus of the stria terminalis. I have seen reports using coronal sections. Others present the distinct nuclei in transverse sections. What is the best...

22 October 2020 3,343 1 View

Can anyone recommend a protocol for prolonged storage of mouse brain tissue for eventual paraffin embedding?

My lab is looking to perform IHC staining on the brains of several dozen mice, and we have a need to store the perfused brains for up to a month before we have access to paraffin embedding. Can...

14 October 2020 6,256 4 View

IHC co-stain, antibodies optimised with 2 different retrieval methods?

Looking to perform IHC co-staining with CD3 (cytoplasmic) and MUM1 (nuclear) antibodies. CD3 polyclonal rabbit, HIER Na citrate pH6. MUM1 is monoclonal mouse, HIER EDTA pH9. I've tried HIER on...

01 August 2020 457 2 View

Is there a minimum optimal diameter for neural stimulating electrodes?

With the 1.27mm diameter of DBS electrodes often leading to the development of scar tissue, and evidence that electrodes with diameters near the size of neurons, approximately 30 microns, are less...

26 March 2020 1,782 3 View

Why this orange hue on my gel electrophoresis?

A new batch of 1x TBE buffer was prepared and I was testing it out (result is attached). I am wondering why half the gel is lighting up orange, as I have never seen this before. I have not changed...

12 August 2019 5,209 4 View

Which post-graduate research textbook would you recommend?

Making a research textbook recommendation to a masters and early doctoral study student - which research text book would you suggest and why?

18 March 2019 1,117 4 View

How can I resolve problems of maintaining tissue integrity during immunofluorescence for paraffin-embedded sections?

18 October 2018 3,208 4 View

How do I determine a proper loading control for my western blot assay using whole tissue homogenates?

I am running a western blot assay using whole tissue homogenates. The homogenates are from collected mouse brains and I am using two groups an Ames dwarf mouse and a wild-type mouse. I see that...

14 October 2018 3,121 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View