Does anyone have a good protocol for taking soil samples and extracting fungal DNA?

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What analysis can be run on an unpaired Likert scale design?

Hello! I am working on securing a grant for a study design on participants' willingness to participate in clinical trials. We were hoping to keep surveys anonymous and have them...

22 February 2024 4,141 4 View

Can I use over the counter Iodine for μCT Scanning?

The μCT facility I'm at mainly works with fossils. So they don't have any Iodine to stain tissue. I was reading that you can use a 1% Iodine & EtOH solution...

06 June 2023 5,660 3 View

Can you perform the Fast Fouriers Transformation in clampfit?

I am looking at local field potentials and brain oscillations in vitro and am wondering if the power spectral analysis can be performed in clampfit. Most manuscripts report using a custom script...

09 February 2023 8,413 2 View

Does anyone have advice for recording local field potentials from acute brain slices?

I am hoping to recording brain oscillations from acute rat/mouse brain slices. I am looking for advice on how to proceed. I want to record local field potentials in the GAMMA and Theta range....

20 November 2022 5,240 1 View

Can action potential phase plots be generated from Clampfit software packages?

Can I generate an action potential phase plot from Clampfit software? If so, how does one do it? If not, how can one begin to produce this plot?

11 September 2022 4,289 4 View

How can I measure afterhyperpolarization potentials using a template search in Clampfit10.4?

Hello-- I have seen some great tutorials for action potential analysis in Clampfit11 but not with Clampfit 10.4. I have included an image of a representative data file and the action potential...

15 June 2022 518 1 View

Why are my M1 macrophages (THP-1) dying after ~5 days post-polarization?

I'm following the protocol (https://www.frontiersin.org/articles/10.3389/fphar.2018.00071/full): Cell differentiation was induced via a 6-h exposure to 185 ng/ml phorbol 12-myristate 13-acetate...

19 April 2022 9,547 3 View

How can I make a brain atlas composite figure in photoshop?

There has got to be an easier way to make the 3 image atlas composite from single atlas photos. Please see the attachments. I am trying to make a figure similar to the attached "atlas 2" from...

17 October 2021 1,648 2 View

What is the preferred dye to verify cannula placement in a SD rat after stereotaxic cannula placement?

I want to find a good dye to verify cannula placement in SD rat brain following stereotaxic cannula placement. Currently, I am trying Chicago Sky Blue (1% ) in saline

01 June 2021 1,126 2 View

Should rats be housed individually following stereotaxic cannulation?

I am performing a series of experiments in SD rats following stereotaxic cannulation. The animals are allowed 7-10 days to recover. Should I be moving the animals to grouped cages (2 rats/cage)...

24 May 2021 6,843 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View