Does anyone have a good protocol for isolation of human ventricular cardiomyocytes?
My article was published in Russian, only the summary of the article is in English. Should I indicate in Research Gate after the title of the article that the article is in Russian?
02 February 2024 8,769 2 View
I propose a joint study on the induction of macrophage polarization in suspension in vitro. The inductor has not been used previously.
17 November 2023 3,630 2 View
Hello there! We are trying to establish sexual behavior analysis of rats in our lab. However, we lack the experience to correctly identify behaviors such as mating, intromission, and ejaculation....
09 May 2023 8,441 0 View
Can I present my journal scientific publications in Russian language for Research Gate? Some of these publications don't have English Summaries.
21 December 2022 6,257 4 View
Can someone recommend the most reliable way to estimate the number of active sites of Ru, Co, and Cu catalysts on Al2O3? 1) Of course there isn't a 100% correct way of doing it, that's why I...
27 October 2020 6,783 5 View
I used auroral electrojet indexes (AE, AU, AL) for the ionosphere modeling. However, there is no data at Kyoto WDC after February 28, 2018 (...
25 August 2020 1,686 12 View
I’m looking for a commercial or free software that allows static fringe analysis (not temporal phase shifting) of interferograms by Fourier-transform method and/or fringe tracing. Currently, the...
15 June 2020 681 0 View
Dear friends and colleagues, I am honoured to announce this Special Issue of Applied Sciences "Nitrogen Fixation by Plasma: Towards Electrification of Chemical Industry". The natural N2...
07 April 2020 2,167 4 View
Could someone help in searching the coefficients for the broadcast GPS model? I was trying to take them from the BRDC navigation files at ftp://cddis.gsfc.nasa.gov/pub/gps/data/daily. However in...
03 October 2019 2,629 2 View
I'm looking for cellular system for analysis of TCR of new species. Something like mouse 54z17 or 58 αβ−/− cells, but without CD4 and CD8.
27 August 2019 5,336 0 View
Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?
23 June 2024 2,264 4 View
We coat the plate in poly-D-lysine (50 ug/mL) followed by fibronectin (50 ug/mL) before plating the cardiomyocytes. After isolating and plating cardiomyocytes from 2-4 day old pups, we fed the...
20 June 2024 393 0 View
I recently isolated mice cardiomyocytes and found some are not individual cells. I tried to stain the cells with Wheat Germ Agulutinin, it did not work. Does anyone have recommendations on the...
20 June 2024 3,225 2 View
Maintaining a WiCell fibroblast-derived line of iPSC in monolayer with laminin coating and E8 media, then using Wnti directed protocol (Gsk3i day 0, Wnti with conditioned media day 3, insulin day...
02 May 2024 6,314 1 View
I have tried isolating ventricular cardiomyocyte by perfusing type II collagenase through adult mice hearts. When looking at the extracted cells under 20X magnification, I don't see the typical...
21 March 2024 3,725 1 View
I conducted the recovery from inactivation protocol to record INa in isolated cardiomyocytes. The very strange thing is that the sodium current couldn't return to the max value (marked 1). The...
14 March 2024 9,544 14 View
What experiments can be conducted to compare the proliferation ability of human induced pluripotent stem cell-derived cardiomyocytes (hiPSCCMs) with a specific gene knocked out using CRISPR-Cas9...
31 January 2024 1,373 4 View
We are looking for a pulse protocol with a prepulse which could eliminate Na+ and K+ currents
23 November 2023 9,833 0 View
Hi! I do patch-clamp on cardiomyocytes in whole-cell configuration, voltage-clamp mode. Everything went well but suddenly the baseline started to drift down (when the electrode is in the bath,...
22 November 2023 4,376 0 View
What is the best cell line for Cardiomyocytes for in vitro functional assay. I need a robust cell line easy to transduce and maintenance. I have several candidate to consider: C21C2, AC16, and...
12 November 2023 2,144 3 View