Does anyone have a good protocol for isolation of human ventricular cardiomyocytes?
I used auroral electrojet indexes (AE, AU, AL) for the ionosphere modeling. However, there is no data at Kyoto WDC after February 28, 2018 (...
25 August 2020 1,532 12 View
Could someone help in searching the coefficients for the broadcast GPS model? I was trying to take them from the BRDC navigation files at ftp://cddis.gsfc.nasa.gov/pub/gps/data/daily. However in...
03 October 2019 2,545 2 View
Ussually, we use running average to detrend total electron content (TEC) data from GPS(GNSS) and to filter required period range. Could someone recomend a paper devoted to comparison of different...
25 October 2018 7,399 2 View
We are working with different fermentation times of silage, when extracted DNA at time 0 yield >100ng/ul but at time 140 days yield
23 July 2018 4,279 3 View
I need something like Paschen curves but for low voltages
10 October 2013 6,327 6 View
I have faced a problem with culturing Ac16 cardiomyocytes cell line. Day 0 I plated 200,000 cells/2ml (well) in 6 wells plate in FGM. Day 3 I put my cells on Low serum medium. When I changed...
24 January 2021 1,456 2 View
I have GD18.5 heart tissue currently snap frozen in -80, apart from mRNA and protein analysis on whole tissue, is there a way of isolating single cell populations to study localization of a...
19 January 2021 4,044 3 View
Hello, I followed a protocol for isolating human cardiac stem cells (Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human...
18 November 2020 2,928 3 View
From the literature I understand that TRP channels regulate Ca2+ signalling in cardiomyocytes and fibroblasts. I want to perform immunocytochemical analysis of TRP channels on cultured cells or...
07 May 2020 6,865 1 View
Hello everyone, Which is the best way to resuspend a culture of adult rat ventricular cardiomyocytes without damaging the cells integrity? This is what I am trying to do. I isolate ventricular...
31 December 2019 1,080 2 View
I have been trying to culture primary cardiomyocytes from neonatal Rat heart using PIERCE Cardiomyocyte isolation kit. I have plated the cells 4 days back and changed the medium after 48 hours....
08 September 2019 8,216 3 View
I am trying to label phospholamban with antibody 2D12 and proteinA-647. Sometimes I get the result below. I don't know what happened.
08 September 2019 9,949 5 View
After 15-20 days of differentation of iPSC to CM I typically passage and plate the iPSC-CM in a 6-well matrigel-coated plate or 12-well if differentiation yielded low number of pure...
05 June 2019 9,673 4 View
I am trying to detect Bin1 in DG75 and IIA1.6 cells. However the several antibodies that I tested all gave different band patterns. Does anyone have any experience with monoclonal/polyclonal...
28 March 2019 7,532 2 View
I have tried western blot and immunocytochemistry for connexins (40,43,45) which are said to be predominant connexin isoforms in mammalian cardiomyocytes. However, there aren't any traces of them...
05 March 2019 365 2 View