I'm looking for cellular system for analysis of TCR of new species. Something like mouse 54z17 or 58 αβ−/− cells, but without CD4 and CD8.
I used auroral electrojet indexes (AE, AU, AL) for the ionosphere modeling. However, there is no data at Kyoto WDC after February 28, 2018 (...
25 August 2020 1,532 12 View
Could someone help in searching the coefficients for the broadcast GPS model? I was trying to take them from the BRDC navigation files at ftp://cddis.gsfc.nasa.gov/pub/gps/data/daily. However in...
03 October 2019 2,545 2 View
Ussually, we use running average to detrend total electron content (TEC) data from GPS(GNSS) and to filter required period range. Could someone recomend a paper devoted to comparison of different...
25 October 2018 7,399 2 View
We are working with different fermentation times of silage, when extracted DNA at time 0 yield >100ng/ul but at time 140 days yield
23 July 2018 4,279 3 View
I need something like Paschen curves but for low voltages
10 October 2013 6,327 6 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...
24 February 2021 9,578 4 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View