I'm looking for cellular system for analysis of TCR of new species. Something like mouse 54z17 or 58 αβ−/− cells, but without CD4 and CD8.
My article was published in Russian, only the summary of the article is in English. Should I indicate in Research Gate after the title of the article that the article is in Russian?
02 February 2024 8,769 2 View
I propose a joint study on the induction of macrophage polarization in suspension in vitro. The inductor has not been used previously.
17 November 2023 3,630 2 View
Hello there! We are trying to establish sexual behavior analysis of rats in our lab. However, we lack the experience to correctly identify behaviors such as mating, intromission, and ejaculation....
09 May 2023 8,441 0 View
Can I present my journal scientific publications in Russian language for Research Gate? Some of these publications don't have English Summaries.
21 December 2022 6,257 4 View
Does anyone have a good protocol for isolation of human ventricular cardiomyocytes?
24 March 2022 8,274 0 View
Can someone recommend the most reliable way to estimate the number of active sites of Ru, Co, and Cu catalysts on Al2O3? 1) Of course there isn't a 100% correct way of doing it, that's why I...
27 October 2020 6,783 5 View
I used auroral electrojet indexes (AE, AU, AL) for the ionosphere modeling. However, there is no data at Kyoto WDC after February 28, 2018 (...
25 August 2020 1,686 12 View
I’m looking for a commercial or free software that allows static fringe analysis (not temporal phase shifting) of interferograms by Fourier-transform method and/or fringe tracing. Currently, the...
15 June 2020 681 0 View
Dear friends and colleagues, I am honoured to announce this Special Issue of Applied Sciences "Nitrogen Fixation by Plasma: Towards Electrification of Chemical Industry". The natural N2...
07 April 2020 2,167 4 View
Could someone help in searching the coefficients for the broadcast GPS model? I was trying to take them from the BRDC navigation files at ftp://cddis.gsfc.nasa.gov/pub/gps/data/daily. However in...
03 October 2019 2,629 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View