I have been trying to find a detailed protocol about staining endothelial cells in the sprouting assays in matrigel. The cells are vascular. I was wondering if anyone had a detailed protocol in regards to how to do this.
Dear Afra,
for immunostaining you can use von Wildebrand factor or CD31 (PECAM) performing standard immunostaining protocol:
fixation 4% PFA, permealization in 0.3%triton, blocking and Primary and secondary antibody.
Good luck
Cesare
I agree with the VWF staining. Here's a link to a vendor:
http://www.abcam.com/index.html?pageconfig=resource&rid=10094
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