Composition of HEPES buffer solution?

Hi All, I want to make HEPES buffer solution and when I search for the composition I could find two different types of it. Can anyone help me which one is the correct method of HEPES...

08 September 2020 450 4 View

Why my HaCaT cells die after thawed?

I tried to thaw my HaCaT cells several times but 99% of them died. They were frozen with 10% DMSO + 10% FBS + 80% DMEM. I also tried 10% DMSO + 90% FBS then they died less but still only 10-20%...

18 October 2019 1,762 2 View

What could be the factors for unreasonably high heat capacity values from DSC measurements?

I have been running DSC for porous materials but the results I got were quite ridiculous. For the solid porous materials, I obtained heat capacity above that of water, which I cannot reason at...

20 September 2019 2,211 3 View

Staining results contradicts PCR identification results?

Hello. I want to ask a question about microbial identification. In our lab we stained the strains to see that is it rod or cocci. After that we sent it somewhere to analyze and identify strains...

21 July 2019 4,460 3 View

Training mice to pay attention to visual stimulus?

Hey all, In a lab I am working in, I designed a behavioral experiment protocol that involves mice being able to freeze in the presence of a specific static visual pattern. I also need them to...

03 July 2019 5,106 5 View

Do you have training courses to make models for my thesis on climate change and food security in Senegal?

Currently I am a student in Master II at the Faculty of Economics and Management of the University Cheikh Anta Diop Dakar.I finish this higher level with a dissertation whose theme is "the impacts...

01 June 2018 8,422 4 View

Seaweed water content.

08 June 2017 5,789 3 View

How can I prepare an optimal nanoparticles suspension?

13 March 2017 2,927 3 View

POWER: For a longitudinal developmental study with 3 time-points, in order to discriminate between trajectories, what is the minimal sample size?

The study is designed to measure executive function in children in 3 consecutive years. The children will come into the lab annually with their parent. Since executive function is itself a highly...

04 October 2015 7,297 3 View

Does anyone have an answer and references materials on the cause of a short term rise in bottom hole pressure in an injection well?

The bottom hole pressure rise in injection wells.

11 August 2014 5,633 2 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How UAV or drone can be beneficial to better estimate crop yield?

As we know, crop yield estimation has been done traditionally with farmer's expert knowledge, deductive sampling in representative areas, and remote sensing with satellite imagery. Farmer's Expert...

01 March 2021 3,468 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View