Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Infinite order of Laguerre?

If Lmn(x) is the Laguerre polynomial of order m and degree n Is there a means of computing Lmn(x) for 1) m goes to infinity 2) n goes to infinity

08 February 2021 6,320 4 View

Sharpening algorithm for similar images with different levels of blurring?

Say for arguments sake I have two or more images with different degrees of blurring. Is there an algorithm that can (faithfully) reconstruct the underlying image based in the blurred...

17 January 2021 7,324 3 View

How to make a pushover Analysis of a reinforced cantilever beam in ABAQUS?

Hello everyone, Im doing a pushover Analysis of a cantilever beam in ABAQUS, made of reinforced concrete, but my model doesnt seem to converge proberly. The program simply aborts before hitting a...

15 January 2021 1,627 3 View

How to illustrate FE Panel Regression with LSDV in a research framework?

First time to use FE Panel Data Regression using LSDV, and not familiar with how to illustrate it in a research framework. We have regional CSR score as IV and Social Development ( four areas:...

14 January 2021 580 3 View

Domestic ancient Egyptian gods and goddesses?

Who are the gods that ancient Egyptians thought of as closer to them and could have a personal connection with them?

25 December 2020 5,505 1 View

Could someone help me recheck my identification of my moth samples?

Greetings to all! I am humbly asking for some help, in regards to the checking of my moth samples. Though it is of great inconvenience to you all, I will respect your decision nevertheless....

08 December 2020 6,009 5 View

Is there a relationship that can be used to relate flow rate (m3/s) to MPa in fluid injection rate?

I am doing simulation exercise of which I have to inject fluid pressure into a borehole. The parameters I am using the injection rate is given in pressure (MPa) and the software accept injection...

01 December 2020 3,221 2 View

What is the current and voltage profile for a short dipole antenna when the monopoles have different lengths?

The textbooks show a triangular pattern for the current as the limiting case for a short dipole, and how this may be considered to be the limiting case for the sinusoidal current distribution....

26 November 2020 5,280 5 View

Best way to teach Data Structures and Algorithms?

Next semester I am teaching an online class on Data Structures and Algorithms. The students will have had two programming classes in C++ and Java each. I am looking for a way to engage the...

11 November 2020 1,176 4 View

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02 March 2021 3,060 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View