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I'm doing a yeast display experiment and have been sorting yeast cells (S. cerevisiae) with a BioRad cell sorter. In the later rounds I've started getting contaminants on the plates. Some plates...
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My BioRad SmartSpec is dying and I need a recommendation for an inexpensive spec for my cell culture room (measuring bacterial and yeast ODs). I don't need something fancy as I already have a...
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One of my colleagues who does a lot of Saccharomyces expression says that His6 tags don't work well for purification of recombinant proteins expressed in S. cerevisiae. Have others run across an...
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Hi all, I want to express a ~100 bp single-stranded DNA sequences in E. coli. The project is to make a molecular beacon that has a ssDNA fragment making a hairpin that unfolds in response to a...
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