I would like to exam enzymatic antioxidant for some cinnamic acid derivatives compounds, so I am planning to use superoxide dismutase assay, so if you have a clear method for this examination including solvent, concentrations, time, temperature, any advice.
Thank you
You can benefit my research by this
Article Some Antioxidant Enzymes in Workers Exposed to Pollutants
Mohamed H Assaleh
yes i have simple methods.
Superoxide dismutase (SOD) activity
The SOD activity was determinated by NBT photochemical reduction reaction method. Centrifuge the homogenated samples , then take 0.1ml the tested enzyme solution into tubes, then add 3.8ml Met-NBT reaction mixture. 0.1ml double-distilled water instead of enzyme solution in the control. Then add 0.1ml riboflavin to each tube. After mix with vortex mixing device, illuminate the samples under a 20W fluorescent lamp. When illuminating the cuvette must keep 3cm from the lamp to make sure well-distributed，then calculate absorbance values in 560nm with a microplate reader.
Utsab Deb Thank you
Abeer Al Sawafi Was a great explanation, Thank you :)
Please find this document which contains the methods of SOD and other oxidative stress parameters but my advice is to find a suitable commercial kit it will be more accurate than other methods and good luck
Abeer Al Sawafi how about the concentration of each solution?
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