Does anyone has an idea to test this reaction on a gel using fluorescent labelling?

More Anjali Pai's questions See All

Can anybody help me with an efficient way to de-protect poly(tert-butyl norbornene-2-carboxylate)?

I want to make poly(norbornene 2-carboxylic acid), for the sake of characterization, I decided to start with the protected monomer norbornene-tert-butyl-2-carboxylate for the ring-opening...

03 December 2020 3,852 5 View

Why Schiff bases change colour upon evaporation?

I got bright yellow coloured Schiff base after 6 hour reaction time. So i kept it for slow evaporation. Solvent was methanol. But after complete evaporation my product colour changes from yellow...

17 November 2020 3,824 2 View

Has anyone observed delamination of pyrolysed SU-8 on SiO2 coated Silica substrates when the pyrolysis is performed outside cleanroom?

We have a quartz tube furnace (diameter 80 mm) outside the cleanroom and when tried to pyrolyse the SU-8 on SiO2 coated Si substrates at 900 C in nitrogen atmosphere, the electrodes looks fine....

08 November 2020 4,357 2 View

Is there any chance for synthesis Schiff bases using 4-methyl-5-imidazole carboxaldehyde?

Is there any chance for getting product using the above aldehyde and different amines.

12 July 2020 8,330 2 View

Can anyone help me finding good impact factor journals to submit uninvited review paper on postharvest chilling injury in fruits.?

I have tried submitting my review paper in relevant journals, but most of them only accept the review paper invited by the editor of the journal

28 June 2020 4,371 5 View

What is an alternate way to recover a polymer product from DMSO or DMF other than evaporation?

Due to the high b.p of DMSO and DMF (189 and 153 degree Celsius), it is difficult to recover my polymer from the solution. I believe extraction in a triple system (such as DCM, DMSO and water)...

04 March 2020 3,028 12 View

What were the active sites on the magnetic activated carbon surface for adsorption of reactive dyes? Were they carbon atoms or Fe3O4?

In the adsorption process by using adsorbent magnetic-activated carbon for removal of reactive blue 13 dye which contains -N=N- group, SO3 group, OH, Cl, etc. In this process what are the active...

24 February 2020 1,110 4 View

Why i am getting only one peak at 280 nm for dyes ?

I prepared aminobenzothiazole reacted with amino phenol azo dyes but on taking UV I got single peak. Why I couldn't get 2 peaks?

18 February 2020 6,949 3 View

Could anyone please help me to get my schiff base product?

Hereby I am explaining the detailed procedure which i done in my lab. 1:1 molar ratio of 2-amino benzothiazole and 4-nitro benzaldehyde were refluxed for 12hr at 100 degree Celsius in toluene...

24 January 2020 5,094 3 View

Is it possible to determine the molecular weight of emulsion polymers by GPC?

I performed an emulsion polymerization of tert-butyl acrylate using KPS as initiator. I confirmed the formation of polymer by NMR. But when I tried to determine the molecular weight using GPC, GPC...

12 January 2020 6,711 8 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Eric A.J. Simko

The approach depends on the purpose of this process. I assume you are using RNase H to remove short RNAs which remain bound at the promoter. Are you removing the abortive transcripts in an attempt to modulate T7 efficiency, or are you trying to analyze the RNAs which remain bound?

If you are looking to remove the Gs from the 5' end of your full-length transcripts to study 5' UTR influence, I don't think RNase H will be appropriate for your application.

Anjali Pai

I am looking to remove the G's from the 5'end of the full length transcripts. I am trying the chimera strategy to remove it.