Does anyone has an idea to test this reaction on a gel using fluorescent labelling?

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any study on protective factors of care givers of dementia and resilience ?

need to review articles on care giving in dementia and challenges faced

12 August 2024 6,424 1 View

WOMAC scale Indian version license to use?

Im conducting a study on knee osteoarthritis with herbal medication and treatment, was hoping to use WOMAC scale but the license seems to be hard to get. Any leads on how to acquire license for...

27 March 2024 5,174 2 View

Do we have anti-corruption index for Indian states?

Index trends in major states by respective anti-corruption effort by Debroy and Bhandari is till 2012 only. is there any index available till date?

26 February 2024 8,820 0 View

How to identify coffe planatation in LULC using GEE supervised classification ?

can we use thresholds ?

12 February 2024 7,207 0 View

Can someone suggest a technique to detect/Quantify DNA hybridization with RNA??

can someone suggest a technique to detect/Quantify DNA hybridization with RNA?? in solution with any analytical methods. Thank You

24 October 2023 6,606 1 View

How can we get data for Halogen oxides (ClO, BrO and IO) present in atmosphere using satellite data?

Are there any specific online sites from where the data for halogen oxides could be accessed?

08 October 2023 4,996 1 View

How layer-by-layer diffusion method is employed to get partial coating of shell over the core?

We synthesized a core-shell magneto-electric nanoparticles for drug delivery application, required layer-by-layer deposition method for the synthesis. How it is employed? What's the exact...

13 September 2023 1,182 1 View

In the synthesis of magneto-electric core-shell (CoFe2O4/BaTiO3) nanoparticles, how layer-by-layer deposition technique is employed?

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13 September 2023 5,871 1 View

Can I know about most absorbance wave length for chlorophyll in coconut trees by using UAV?

I need to know about the absorbance wave length in chlorophyll.

13 August 2023 3,066 1 View

If some fertilizers shows increased iron uptake, germination and overall vigor which antioxidative defense enzyme should be targeted ?

The nanofertlizers were applied and rationale of augmented vigor is needed.

18 July 2023 7,082 3 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

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07 August 2024 6,034 3 View

E.coli contamination in human RNA seq data ?

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How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

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06 August 2024 726 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Low-yield gel extraction problem?

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05 August 2024 9,798 3 View

The Optimal Experimental Approach for Gene Function Analysis?

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04 August 2024 6,265 1 View

PCR showing no bands - Master mix and primers didn't mix?

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31 July 2024 2,406 6 View

How to check pH of a high strength hydrogel?

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30 July 2024 942 2 View

How to we see transcription activity of the bhlh79 family is at the N-terminal or C-terminal?

I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...

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Eric A.J. Simko

The approach depends on the purpose of this process. I assume you are using RNase H to remove short RNAs which remain bound at the promoter. Are you removing the abortive transcripts in an attempt to modulate T7 efficiency, or are you trying to analyze the RNAs which remain bound?

If you are looking to remove the Gs from the 5' end of your full-length transcripts to study 5' UTR influence, I don't think RNase H will be appropriate for your application.

Anjali Pai

I am looking to remove the G's from the 5'end of the full length transcripts. I am trying the chimera strategy to remove it.