Does anyone has a sequence of plasmid pCMV-BICEP-4?

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Is there a link between age of voters, political preference and personal savings and investments?

I am searching for papers that help me answer this question. Furthermore, I need studies that analyse the saving behaviour of polically polarized persons (far left and far right). e.g. do far...

26 July 2024 7,780 0 View

Determine the inside temperature of an inside of a barrel using mica band heater so that it reaches 170-180°C?

The following are given: Thermal Conductivity of Barrel Material of 37.67 W/(m·K), Barrel Length of 290mm, Barrel Inner Diameter of 16mm, Barrel Wall Thickness of 9.5mm, Barrel Outer Diameter of...

22 May 2024 4,296 1 View

Determine the inside temperature of an inside of a barrel using mica band heater so that it reaches 170-180°C?

The following are given: Thermal Conductivity of Barrel Material of 37.67 W/(m·K), Barrel Length of 290mm, Barrel Inner Diameter of 16mm, Barrel Wall Thickness of 9.5mm, Barrel Outer Diameter of...

21 May 2024 6,410 1 View

What are the methods available to test the High-Temperature & Ultra High-Temperature Composite (Materials)?

Folks, I need an answer for the methods available to test the High-Temperature & Ultra High-Temperature Composite (Materials), Especially in Aerospace Applications.

14 April 2024 8,681 1 View

Which are the latest Additive Manufacturing & Casting Techniques used for Aerospace Applications?

Dear Professors/Researchers, Can you please suggest, the latest Additive Manufacturing & Casting Techniques used for Aerospace Applications?

14 April 2024 3,713 1 View

Which is the best coating/Surface treatment/Processing technique available to minimize Nozzle erosion?

Which is the best coating/Surface treatment/Processing technique available to minimize Nozzle erosion in Hypersonic vehicles?

14 April 2024 8,694 2 View

Are there regulatory requirements for the design and organization of rooms for clinical NGS laboratory?

06 February 2024 1,208 1 View

What is the BEST smartphone APPlication for Augmented Reality in Neurosurgery?

Do you use any Smartphone App for AR in Neurosurgery?

24 January 2024 5,746 4 View

How to model evaporation of a sessile droplet with pinned contact line in Comsol Multiphysics?

I am trying to model evaporation of a droplet on a substrate in Comsol. I've managed to reproduce the methodology of the tutorial model "Droplet Evaporation on Solid Substrate" and it works fine....

13 December 2023 8,914 0 View

Is it possible to complain on scientific journal?

Is it possible to complain on scientific journal? For example, if the paper was initially accepted by the editor (after passing 2 rounds or review) and later rejected by the same editor for...

11 November 2023 6,674 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View