Does anyone associate what kind of mold?

More Aleksandra Wójcik -Mészároš's questions See All

HPLC baseline problem. Baseline problem?

Hello, I noticed a problem with my HPLC analysis and wanted to ask for help. During my analysis I've noticed repetitive problems with the baseline: it is dropping and then (usually) going back...

20 May 2024 6,501 3 View

What’s are microorganisms adaptation to live in high sugar concentration (osmophile microorganisms)?

Hello I am looking for information about osmophilie microorganisms and specifically their enzymes. If there are any industrially important enzymes from microorganisms living in environment where...

19 May 2024 136 1 View

How to do root sampling for transcriptome analysis?

I am preparing an experiment in which I would like to sample roots of sunflowers grown in pots (substrate is soil) in later stages of the plant development. Can anyone tell me, what is the best...

29 April 2024 8,775 1 View

What's the specificity/tolerance of TaqMan probes for differences in sequence?

Hi, Does my TaqMan probe recognize the gene only if the sequence has 100% coverage with the probe or will I also get signal if there is a SNP or even a few bp difference? And if a few bp are ok...

09 April 2024 6,856 3 View

Why ions affect enzyme activity different in differ buffer?

I have tested enzyme activity (b-galactosidase) with cation ions in different buffer and the results differ dramatically. For example, while I used MES buffer calcium acts as an activator but when...

11 February 2024 3,135 2 View

Cell lines from Accegen - quality?

Hello, Does anyone have any kind of experience with ordering cell lines from Accegen? I'm wondering if they are trustworthy and about the quality of provided cell lines?

26 November 2023 3,757 1 View

Do you know of any tourism journals that have a mentoring programme for beginning reviewers?

I think that mentoring programmes support in meeting publishers' expectations.

05 October 2023 2,584 1 View

How do you get information about the number of chromosomes or DNA content?

I need to get information about the number of chromosomes or DNA content. I have seedlings from in vitro that were frozen at -20 degrees after liquidation of the culture. They are seedlings from...

28 September 2023 6,373 2 View

Detection of phosphorylated transmembrane proteins with WB?

I need to detect phosphorylated IGF1R in cell lysate and brain lysate with Western Blot. Are there any life hacks for detection of such proteins?

05 September 2023 7,310 0 View

Would you like to attend our Summer School on Energy and Society?

https://webmagazine.unitn.it/en/evento/sociologia/115480/esa-rn12-environment-and-society-summer-school

10 March 2023 3,806 3 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Help me to identify these fungal slides?

These fungal slides are isolated from cultured Bread mold on PDA media and smear is treated with Lacto phenol Blue dye ..

13 July 2024 9,701 1 View

Should all lactic acid bacteria be catalase negative? should all probiotic bacteria be catalase negative?

I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...

12 July 2024 3,756 4 View

Can we change blood agar in the mCIM and eCIM protocols?

In the modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) protocols, can we replace blood agar with another culture medium?

10 July 2024 6,352 0 View

Why do we use Horse Serum for C2C12 cell differentitation?

I have seen some papers that differentiate C2C12 myoblasts into myotubes by changing the growth medium that contains 10%FBS by a medium containing 2%Horse serum/calf serum + 1% NEAA. What's the...

04 July 2024 4,732 1 View

Arabidopsis contamination in Hydroponic Culture ?

Hi I have a real problem growing Arabidopsis in hydroponic culture .I am using the bvoxes from Araponics and a Binder growth chamber with short day conditions and everything accurate .the seeds...

25 June 2024 9,151 6 View

Why I am not getting colony after transformation in Dh5alpha?

I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....

19 June 2024 6,404 4 View

Why don't my intestinal organoids from children's polyps grow?

Hi! I am having trouble growing intestinal organoids from children's polyps. Sometimes they don't grow at all, and other times they look dark and unhealthy, as shown in the attached pictures...

18 June 2024 4,781 3 View