I have been using standard volume of 10 µl vectashield-DAPI for cells stained for confocal microscopy expts. I am still getting the inadvertent cytosolic staining.
I suggest to do it separately:
1.stain with DAPI (0.5 microgram/ml; 1-2 min is enough)
2.wash once
3. use the anti-fade
I am working on FTC for handling sensor faults in the system. I have read about observer-based FTC schemes which use error threshold-based logic between the states and estimated states. If a...
10 January 2021 5,771 2 View
I want to know whether a luenberger observer or a modified form of it be effective for estimating the states of a system of the following forms: 1. x_dot = f(x,u,d); y = x 2. x_dot = f(x,u); y =...
20 December 2020 1,392 4 View
I have a time series of two rainfall products. For error decomposition analysis, I have to calculate HB, MB and FB. Is there any software for calculating these variables.
27 November 2020 980 2 View
I want to target particular protein/peptide/enzyme by cell culture methods. I would also like to know screening methods for cell cultures to analyse and diagnose the drug effect on particular...
24 November 2020 7,255 5 View
I am working on voltage control of a Standalone DC Microgrid consisting of Single PV source, a battery and loads. The PV is interfaced through Buck converter to the DC bus, the battery is...
01 October 2020 7,071 2 View
How can we make the rating of a battery in MATLAB/Simulink to Amp-sec instead of Amp-hour? I have tried with reducing the factor of 3600 to 1 inside the battery block. Is this approach completely...
03 July 2020 4,017 4 View
Various colorimetric assays we use are blue/purple/pink/red but not in green shades. Is there any particular reason for this?
30 May 2020 5,834 1 View
Please suggest me a good source to learn the design of adaptive controllers like MRAC. Resources related to the implementation on MATLAB platform and for Power electronic converter systems would...
01 May 2020 7,422 3 View
What are various options available while determining the binding affinity of a protein to it's target? In my case the target is also a protein. Are there any standard physical methods to...
09 March 2020 8,053 8 View
Dear all, How to calculate setup time and hold time of a memory circuit in CADENCE Spectre Simulator?. We have designed Content Addressable Memory (CAM) using 45 nm technology in CADENCE Spectre...
05 January 2020 9,460 3 View
Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru
02 March 2021 1,787 2 View
02 March 2021 3,060 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.
02 March 2021 7,735 3 View
Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...
01 March 2021 3,052 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View