Does anybody suggest to reduce cytosolic staining along with nucleus in the cells stained with vectashield-DAPI reagent for fluorescent micrscopy?

27 December 2013 1 8K Report

I have been using standard volume of 10 µl vectashield-DAPI for cells stained for confocal microscopy expts. I am still getting the inadvertent cytosolic staining.

Gal Haimovich

I suggest to do it separately:

1.stain with DAPI (0.5 microgram/ml; 1-2 min is enough)

2.wash once

3. use the anti-fade

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