Venkata Raghuram

2 Questions 9 Answers 0 Followers

Questions related from Venkata Raghuram

Can anyone quantify IL-6 or TNFa expression from immunofluorescence microscopic images of cultured cells?

Is image J software the right one or is some other software helpful?

13 April 2014 3,331 6 View

Does anybody suggest to reduce cytosolic staining along with nucleus in the cells stained with vectashield-DAPI reagent for fluorescent micrscopy?

I have been using standard volume of 10 µl vectashield-DAPI for cells stained for confocal microscopy expts. I am still getting the inadvertent cytosolic staining.

27 December 2013 7,704 1 View