Venkata Raghuram

4 Questions 10 Answers 0 Followers

Questions related from Venkata Raghuram

Can anyone please let me know how to prepare metaphase spreads from cultured CD4 and CD8 lymphocytes invitro?

We isolate and sort the cd4 and cd8 population from whole blood and enrich these population by further culturing these cells in vitro by adding mitogen PHA followed by colemid addition (10ug/ml)...

13 June 2019 4,427 0 View

Can anyone quantify IL-6 or TNFa expression from immunofluorescence microscopic images of cultured cells?

Is image J software the right one or is some other software helpful?

13 April 2014 3,380 6 View

How does an external oxidising agent (chemical) undermine the mitochondria of the recipient cell?

Is it through endocytosis by means of vesicular transport system?

02 January 2014 8,364 1 View

Does anybody suggest to reduce cytosolic staining along with nucleus in the cells stained with vectashield-DAPI reagent for fluorescent micrscopy?

I have been using standard volume of 10 µl vectashield-DAPI for cells stained for confocal microscopy expts. I am still getting the inadvertent cytosolic staining.

27 December 2013 7,750 1 View