Which are the most recent implications of ESL Academic Discourse in language teaching?

Language teachers at UTP (Language Department) are increasingly interested in the role of academic papers in the development of language (English) teaching practices. Research papers in Academic...

22 February 2021 1,388 4 View

How to analyze data from an augmented design for locations x genotype interaction?

I have data of multiple environment trials (MET) , the design used was an augmented design in each of the locations. And I would like to analyze the data with Rstudio or a free software. Does...

06 November 2020 6,084 3 View

Does anyone know of an open access journal indexed in JCR with no publication fee?

I wonder if anyone knows a dream journal that meets all the following 3 criteria: indexed in the reputable Journal Citation Reports open access publication free of charge Our research falls...

23 January 2019 2,084 10 View

Do I need to purify labeled primers to sequence microsatellite with a fragment analyzer?

To detect microsatellites using primers labeled in applied biosystems platform. I will use multiplexing (3 dyes) same specie. I need to make it as cheap as possible, in sequencing equipment (serie)

01 September 2018 4,539 3 View

Why would you use a non-parametric test such as Kruskal-Wallis test instead of a parametric test such as ANOVA?

We are conducting a research in the field of Behavioral Finance where some of the papers that we consulted have used the Kruskal-Wallis test for testing mean differences among groups. However we...

15 August 2018 1,385 4 View

Aside from M9 Minimal Media, what are other types of minimal media?

My partner and I are trying to culture bacteria. We are attempting to culture bacteria in minimal media using plastic as its carbon source. We would like to know an alternative in case we do not...

07 May 2018 2,413 3 View

I wonder wether can we evaluate species habitat preference through environmental niche modeling tools?

I got an idea about this knowing beforehand an specie (Plecturocebus caquetensis) which, due to lack of ecological and biology knowledge, its recognized preference is related to inundated habitats...

27 December 2017 7,335 3 View

Anyone can supply the results and information related to the assessment of ecosystem services in the Colombian Orinoquia?

Dear all, I am interested in the results of the Alexander Von Humboldt Institute in Colombia investigation which was about identifying and mapping ecosystem services in the Colombian...

06 March 2017 1,380 3 View

Which is the best way to re-arrange data before running the analysis on DISTANCE?

31 August 2016 5,438 11 View

Are there any other tracking methods aside from satellite tagging that are just as efficient in tracking the long distance movements of whale sharks?

The migratory patterns of Rhincodon typus is very interesting to me and I have read that satellite tagging is the method typically used when tracking their movements. However, I would like to know...

07 July 2016 3,382 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View