Does anybody know what is the difference between SAP and FastAP and their impact on PCR-purification prior to Sanger sequencing?

Sonia Rocío Quintanilla @Sonia-Quintanilla

19 September 2017 1 949 Report

Hello everybody, I used FastAP (instead of (Shrimp Alkaline phosphatase)) and Exonuclease I for PCR purification prior to Sanger sequencing. Despite is very useful for short fragments (

Xinglin Jiang

primer dimers could be the reason

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