Xinglin Jiang

2 Questions 13 Answers 0 Followers

Questions related from Xinglin Jiang

What is the most sensitive reporter gene in bacteria, for example E.coli?

I need to monitor the expression of some genes or gene clusters in E.coli or Corynebacterium or Streptomyces. we plan to insert a reporter gene after our target promoter or the gene or the operon....

02 November 2019 406 0 View

In pcr, are the templates double stranded or single stranded at 72oC extension?

1) 72 oC is low enough for a long primer to bind to single stranded template, so it should not be a problem for the template to turn back to double stranded form (as it have even higher Tm). 2)...

13 March 2019 2,388 4 View