I would like to know the viscosity of Matrigel (Corning) as function of temperature. I already know that it solidifies at 37° and its liquid at 4°, but i would like to know what's the transition form, and what happens with different Matrigel concentrations.
Thanks in advance,
Simon
Jeroen Rouwkema
Given that matrigel is derived from a biological source, in my experience you can also expect batch-to-batch variation in the gelification behaviour. Keep this in mind if your aim is to do a precise quantification.
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Ismet Caglar Tanrikulu
I had put some of the Matrigel I was culturing my HUVECs on on a rheometer a while back, and it had produced a pretty flat G'/G'' from 20ºC to ~40ºC. I don't exactly remember the details, unfortunately.
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