Try looking in this article

http://www.nature.com/nri/journal/v12/n4/fig_tab/nri3175_T1.html

You could use LY6C. If you are doing a staining on tissues CD11b/LY6c could be fine to identify monocyte population in there.

I mean a double staining with CD11b and Ly6c.

Anti-Mouse CD115, also known as macrophage colony -stimulating factor receptor (M-CSFR), is specifically expressed by cells of monocytic lineage and by progenitor cells.

CD68? However, localised to intracellular granules.

Ah, I forgot to mention F4/80....

Are you looking to track the cells in-vivo or simply ex-vivo flow markers?It is very difficult to (without labeling in the blood or bone marrow) to differentiate a monocyte from a tissue macrophage during inflammation. This is because the monocytes are differentiating into macrophages once they leave the blood. We used a number of different methods in this paper (Ly6c+ "inflammatory monocytes" are microglial precursors recruited in a pathogenic manner in West Nile virus encephalitis. 2008, Journal of Experimental Medicine). Thisinclude GFP-CFMS, micro particle labeling and simple adoptive transfers.

Ly6C incombination with F4/80 and LY6G and CD11b can be used.

Monocytes are LY6G- LY6C+ CD11b+, and F4/80 dim, Tissue Macs from spleen, Peritoneum and lung do not have LY6C and stain for F4/80( bright in lung and peritoneum, dim in spleen). LY6G is the neutrophil marker

John, not all monocytes are ly6C-, so your panel would not work. Actually a majority of circulated monocytes are Ly6C-, only around 10-15% of blood monocytes express Ly6C--

Daniel,

I agree that some blold monocytes are LY6C negative and CD115 could help with their identification. However, the data suggests it is a minority, about 28% of blood monocytes which are are LY6C negative(Glasgow cytometry part A 69A:281-290). The best available evidence shows that as the monocytes lose LY6C they are maturing, with LY6C dim monocytes having different chemokine markers than LY6C bright monocytes. The thinking is that the LY6c dim monocytes lose LY6C while circulating and enter tissues as LY6Cdim monocytes. The LY6C negative monocytes have only recently been described and their steady state behaviour has not been described. Rather than calling any blood cell as a moncyte, I am using the expression of LY6C to define monocytes. It is a bit loose perhaps but.....

John,

I agree with your position regarding what happens with Ly6c monocytes, indeed we published on this in 2008 in JEM and have more work on this exact premise coming out in a decent journal in a few weeks. However, the monocyte compartment is heterogenous, and Giessman, Jung, Ginhoux and Randolph have all shown independently the differential capabilities of LY6C hi and lo monocytes. Furthermore, we have shown using transgenic CD115 mice and CXxCR1 mice the different capabilities.

Overall the primary issue is we have not spent enough time characterizing the blood. In theory t and b cells are monocytes (using a pathologist definition which is based of the histological characteristics). In humans there are at least 3 subsets of monocytes.In mice my guess is we will find 3-5 different myeloid monocyte subsets with different capabilities.

CD64 is also a good monocytic marker. we have gud experience of it. u can look for this.

F4/80