Does anybody know of a plasmid or DNA vector which lacks or has a single TTAA site?

31 March 2014 1 237 Report

As it is a 4 bp sequence, it frequently occurs in most plasmids. If anybody knows of such plasmid lacking or having single TTAA site?

Ravinder Kumar

Dear Oliver

I have a restriction enzyme cutting (probably) at the TTAA site but to prove it I need a fragment containing single TTAA site and sequence the fragments generated with my enzyme using forward and reverse primers so as to find the actual cutting site. Anyway I think using primers for amplifying such a fragment from any DNA will work.

Thanks for concern.

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