Ravinder Kumar

7 Questions 12 Answers 0 Followers

Questions related from Ravinder Kumar

How can we map the promotor sequences of a bacterial protein from 5'-UTR of the coding DNA sequence?

What programs can efficiently do this job and can the bacterial promotors be very far away (more than -50) from the first amino acid?

16 February 2015 2,980 5 View

Can we sequence DNA on sequencing gels using fluorescent labels?

Can fluorescent labels be detected with a phosphor imager? We have Fujifilm- FLA 9000 and Biorad Screen Eraser-K set up for detection.

24 November 2014 9,139 3 View

Does anybody know of a plasmid or DNA vector which lacks or has a single TTAA site?

As it is a 4 bp sequence, it frequently occurs in most plasmids. If anybody knows of such plasmid lacking or having single TTAA site?

31 March 2014 222 1 View

How can we elute protein bands from native PAGE so as to check activity?

I want to elute active protein from native gel and still retain activity. Can it be done? Any reference would be helpful.

28 February 2014 1,039 9 View

Can coomassie stained gels which have been destained, be stained again? With coomassie stain and silver stain?

I have stained my SDS-PAGE with coomassie blue but bands are very light. Probably, the stain was not good. Can the gel be stained again with new coomassie or silver stain? Will the staining be...

12 February 2014 8,914 11 View

Do proteins retain activity after acetone precipitation?

I need to concentrate my protein samples for running SDS-PAGE as well as checking activity.

05 February 2014 5,768 4 View

What is the minimum amount of protein that can be easily visualised on PAGE by coomassie blue staining and silver staining?

I have a purified enzyme which is giving good activity on its substrate but I am not able to visualise any bands on SDS-PAGE gels. How much is the minimum of protein am I need to load?

29 January 2014 177 18 View