Krish Dodani

4 Questions 1 Answers 0 Followers

Questions related from Krish Dodani

Does anybody know of a cell type with enriched expression of CD63, CD9 and CD81?

I need an appropriate positive control for WB antibodies I am using to confirm the presence of exosomes in samples. The thing is I am having reproducibility issues, and I would like distinguish...

20 June 2024 4,172 3 View

How long do you leave endothelial cells to grow on Transwells before performing macromolecular permeability assay?

I want to test the effect on endothelial permeability of certain compounds. I am using Transwell filters (0.4 microns pore size), FITC conjugated dextran (40 kDa) and Human Aortic Endothelial...

21 December 2023 7,606 1 View

Is it normal to see floating particles that resemble unattached cells in adherent cell culture?

I am culturing Human Aortic Endothelial Cells from ThermoFisher (Cat #: C0065C). I am doing it with the media and supplements they sell for it, which are called M200 and LVES respectively. Despite...

18 October 2023 9,166 1 View

What is my best option to concentrate a protein solution obtained through RIPA lysis buffer?

I recently performed protein extraction with RIPA buffer of some cells I collected. The intended purpose was to use it to load in an SDS PAGE gel for subsequent Western Blot. I made the mistake of...

21 September 2023 7,603 4 View