Does anyone have experience using High Molecular Weight DNA Extraction Kits for extracting DNA from plant leaves?

Hi there, I'm looking for a High Molecular Plant DNA Extraction Kit for subsequent Nanopore sequencing. Does anyone have experience using commercially available kits? Any...

03 January 2021 9,487 4 View

Is possible that an antibody drug conjugated (ADC) against KLH-fluorquinolone has a strong effect in T cells viability?

I have a question for KLH experts. Is possible that an antibody drug conjugated (ADC) against KLH-fluorquinolone has a strong effect in T cells viability? Is a T- cell expressing KHL- or...

24 September 2020 2,931 1 View

What is the best way to extract Spike protein from whole cell lysates for ELISA ?

Hello wise scientific community, I am trying to measure the concentration of Spike Protein ( whole protein) expressed by my cell line transfected with a pC DNA 3.1. I can see a nice band by...

17 September 2020 7,265 4 View

Does anyone know about public databases of baseline corticosterone in reptiles?

I'm searching some public databases of baseline corticosterone in reptiles, I know there is a public database of hormones by Vitousek et al. 2018 ( https://hormonebase.org/ ) and has some data...

31 July 2020 540 3 View

IEEE 34 node test feeder in DigSilent PowerFactory?

18 April 2020 1,949 2 View

Paired-test or unpaired-test?

I have a group of PDXs models derived from the same patient. I want to know if the results between differents mice with the same treatment have significant difference or not. The analysis of 3...

24 March 2020 7,004 4 View

How to associate parameters to function switch?

I am using comsol to calculate the IV curve using a physical model. The problem is that I am using the switch function to evaluate multiple spectra but I can not associate a temperature for each...

30 June 2019 2,894 1 View

Why in the simulation of a fracture toughness test, the values of stress intensity factor are not uniform along the crack of the specimen?

In the virtual simulation of toughness using a standard steel specimen (A516-Gr.70), the maximum equivalent (Von-Mises) stresses are uniform along the entire length of the crack. However, when the...

21 January 2019 3,694 1 View

Does anyone know an alternative co-stimulation signal to feeder cells or soluble CD28 for CD4 T cells?

I want to evaluate the CD28 loss on CD4 T cells in culture with different stimuli. I used to stimulate this cells with anti CD3/CD28 dynabeads but actually i don't know if it could interfere with...

31 December 2018 2,629 3 View

Can someone tell me where can I find the theory of anxiety postulated by Zung or the original manual?

I need it to write my thesis on anxiety and maternal attitudes in mothers in prision.

11 September 2018 9,452 1 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Which are the journals for free paper publication?

A bunch of flower for everybody. I would like to ask about the list of journals which we can publish our paper for free. Thanks in advance. Sincerely Walid

25 February 2021 9,790 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Hello, does any one know a free medical text dataset?

a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards

22 February 2021 5,988 2 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Can anyone recommend which is the best free questionnaire software please?

Hello, I am looking to send out links to an online survey. My previous research project used a paid for survey software. I am now looking for something that's free but straight forward to...

19 February 2021 5,641 3 View