I want to check if some peptides may induce the expression of IL-12p70 in murine splenocytes DCs.
CD11c will give you a very broad look at DC populations, yes. If you can spare one more channel, I'd add CD11b as well, so you can differentiate myeloid and lymphoid DCs (lymphoid are CD11c+ CD11b- and myeloid are CD11c+ CD11b+).
That said, the DC world is complex, and differentiating a given DC population from other DCs (and from macrophages and monocytes) by flow cytometry needs fairly elaborate antibody staining. So to start, CD11c alone will tell you whether DCs are responding to your peptides, and CD11c & CD11b together will tell you whether all DCs, or specifically lymphoid/myeloid are responsible for whatever phenotype you see. Just be aware that once you know which DC family is responding, there might be follow-up questions you will need to answer (is it inflammatory DCs? CD8a+ DCs? Plasmacytoid DCs? etc.)
Good luck!
Thank you Andrew! I have no experience with spleen DCs. I'll first perform one experiment with CD11c and CD11b for gating and checking for the expression of some markers. Then, if they respond, I'll look more deep into other subsets with a more broad panel of antibodies. Thank you! Do you have any publication to suggest?
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