Dear Muneeb
In order to correlate SPAD readings with actual chlorophyll content, firstly you have to construct a calibration curve for the plant species you are interested.
You have to record the SPAD measurements from leaves of different greenness (I think that 30 leaf samples are quite enough) and then you have to estimate their chlorophyll content with a biochemical method.
Then, the calibration curve of SPAD readings versus leaf chlorophyll content can be used for chlorophyll content estimation using only the respective SPAD readings of the unknown samples of the certain plant species.
Dear Muneeb
In order to correlate SPAD readings with actual chlorophyll content, firstly you have to construct a calibration curve for the plant species you are interested.
You have to record the SPAD measurements from leaves of different greenness (I think that 30 leaf samples are quite enough) and then you have to estimate their chlorophyll content with a biochemical method.
Then, the calibration curve of SPAD readings versus leaf chlorophyll content can be used for chlorophyll content estimation using only the respective SPAD readings of the unknown samples of the certain plant species.
thanks sir, can i compare the spectrometric readings with the SPAD readings, or that can directly give me the actual chlorophyll content ?
According to the certain biochemical method that you will use, you must convert the spectrophotometric measurements to chlorophyll concentration (for example mgr/gr fresh weight, mgr/cm2, mgr/gr dry weight) in order to construct the calibration curve.
In the attached paper you can see one of these methods.
thanks alot sir, sir i had done my research on photosynthetic partioning in asiatic lillies, please suggest some journals where can i publish my paper, also suggest a research topic for me in case of doctoral programme of floriculture
Dear Muneeb
I am sorry but considering that floriculture is not in my area of expertise I cannot give you any relevant advice.
Good luck for your research!
thanks mam,
plz tell me can i get the schlorship for phd horticulture in floriculture at your university
You cannot use SPAD meter for estimation of chlorophyll content in leaves because it gives us only a realtive measure of greenness and it cannot help us to convert the readings to absloute amount of chlorophyll content.
Suppose you one to measure chlorophyll content in rice at vegetative stage, take 30 leaves. Measure SPAD value and measure chlorophyll content by standard procedure. Do regression analysis. You can easily come to mg chlorophyll per g f.w. etc. Otherwise report as SPAD value. There is one problem with SPAD meter. Leaf thickness influences the reading. One should be careful about it.
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