I would like to over produce cysteine in E. coli, but it might be that cysteine is toxic in higher concentrations. Anyway, cysteine is commercially available and has to be produced somehow. Probably in E. coli.
Thank you for the PDF Christian. If I got it right, a transporter is important for higher (extracellular) cysteine production. Are their other means to increase the intracellular cysteine concentration?