Does anybody have the following preserved tubers?

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How do I culture primary hemocytes from lepidoptera?

I would like to establish a hemocyte cell line: Do you have any protocols?

19 January 2015 5,405 3 View

What is corrct way to define ports when simulating FSS and surface waves in CST?

I need to simulate a mushroom FSS and I'm struggling with the ports. What's the correct way to define them? Should I construct a proper SMA connectors, or is it enough to put a waveguide ports...

19 January 2021 6,590 4 View

Can anyone identify this fungus/mushroom from China?

The pictures were taken in Guishan Mts in Yunnan. The fungus is ca. 10-12 cm high.

07 January 2021 6,639 10 View

Can forward and reverse sequences of PCR products be entirely different from each other?

Dear All, The forward and reverse sequences obtained from the PCR products are not identical at all. Can I still make the contigs for doing BLASTn? Individually both sequences are showing the...

22 December 2020 5,280 5 View

Hi Is there any one, who help me in processing my raw data files of some mushrooms of LC-MS , by using MZmine2, MS- Dial etc ?

I have received some of my LC-MS raw files but I can not process it by using MZmine2. MS Dial, for compounds detection. Thanks in advance.

02 November 2020 3,564 5 View

In this sterile needle biopsy wet-mount, does the motility pattern fit with Oomycete zoospores?

We are examining tissue from a cluster of human and canine patients with a similar pattern of systemic illness of unknown cause. All members of the cluster have evidence of motile zoospore-like...

23 October 2020 4,480 3 View

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mushrooms play a vital role in human life used for food, medicine, and chemical industries, many edible mushroom-like morels can not be grown by a human, and this type of mushrooms are called...

18 October 2020 1,457 12 View

Complete protocol for Fungal Genomic DNA isolation?

Dear all, I am looking for recommendations for an efficient protocol for the extraction of Genomic DNA from several fungal cultures. I have most of the chemicals and equipment used in the process,...

20 September 2020 7,906 7 View

Is there a valid alternative way to replace the use of camera lucida (or drawing tube) for scientific and taxonomic drawings?

So far I have not been able to find a camera lucida that fits a low range microscope.

05 September 2020 3,342 4 View

What are the equation and conversion factor to estimate protein content from mushroom?

Total nitrogen was calculated with Kjeldahl method, I found different equations and factors ''Total nitrogen content of shrimp waste was determined using the Kjeldahl method. Separately, pure...

17 August 2020 4,572 9 View

Does it matter what order of extraction solvent is used for extracting bioactive compounds from medicinal mushrooms (elaborate description below)?

Hi, My question is regarding medicinal mushroom extraction, but may also apply to broader extraction knowledge of all biota. I understand, that water and ethanol as extraction solvents (along...

25 June 2020 1,113 9 View