Does anybody have some experience in western blot analysis of cytochrome c release?

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Emilia Joanna Orzechowska

Or maybe you can recommend another quite fast and non-problematic method for identification of mitochondrial pathway of apoptosis activation?

Lisa Saxena

Yes I am looking for cytochrome c release in human breast cancer cells. I would recommend you to go for Santa Cruz antibodies for the same. They are quite good. Happy research :)

L. Miguel Martins

For quantitative analysis of CytC release I would recommend an ELISA assay. You can google "CytC ELISA". Check also some articles by Luca Scorrano.

Thanks a lot Lisa Saxena, Dilruba Ahmed and L. Miguel Martins! I will be trying with western blotting at first but I am truly gratefull for other ideas and tips (which can be really important if WB will not work) :)