Is there a dataset of atmospheric river tracks for the whole continental US?

I'm looking for the period 1960-2010, so the dataset would most likely have to be based on re-analysis data. Most things I found are for the West Coast only and often just feature the landfalling...

18 January 2021 3,466 2 View

Where to publish my methodological working paper?

Recently I decided to switch my PhD project to a cumulative form. I have already written an extensive methodological chapter about my adaptation of interpretivist methodology and ethnographical...

11 January 2021 5,337 6 View

How is single molecule's fluorescence lifetime depending on excitation density?

It is known, that the fluorescence lifetime of an ensemble of molecules being in close vicinity can change, as intermolecular processes between excited chromophores (e.g. superradiance,...

28 October 2020 7,656 3 View

Minimum participants for a focus group interview ?

I am involved in qualitative research and at the time working on the methodological background. My colleague, which was doing the interviews, brought to me they did some focus group interviews....

30 March 2020 4,000 16 View

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10 June 2019 6,662 4 View

How to mesure fluorescence excitation intensity under microscope ?

Hi everyone, My problem is quite simple: I would like to get the fluorescence excitation intensity in mol.m-2.s-1 (or W.m-2). And I didn't find any protocol/advice online to measure it. I...

15 January 2019 9,583 2 View

What insulin receptor antibodies do you recommend for WB and ICC on rat neurons (Santa Cruz sc-710 and sc-711 aren't sold anymore)?

My stock of sc-711 is gone, and SC has been prohibited from selling polyclonal antibodies. I want to do both WB (could be either IRalpha or IRbeta) and ICC, especially unpermeabilized cells (so...

15 January 2018 2,474 3 View

Who knows talented prospective PhD students who want to pursue an academic career in sustainability science?

27 April 2017 2,147 2 View

Do you know of studies that compare the proactiveness of environmental strategies of suppliers with those of OEMs (in the same sector)?

28 April 2016 1,121 1 View

Which method should we use for analyzing a sample of 45 companies at two points in time?

25 February 2016 1,868 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View