How to analyse LC-MS spectra of extract and identify compounds?

I have LC-MS spectra of extract where I have the PDF format of the spectra. I sent my extracts to a company who provided me with the pdf only (Not the original file of the software). The library...

02 March 2018 3,157 4 View

Does cDNA synthesized using a cDNA synthesis kit for Real Time PCR use works in End Point Conventional RT-PCR?

I have synthesized cDNA from 1ug of RNA using Quantitech Reverse Transcriptase RT Kit from Qiagen which is for Real Time PCR use. But in out lab we have Conventional RT-PCR. Can i use this cDNA...

07 August 2015 3,636 12 View

How can a mixture of crystals obtained from plant extract can be separated?

I am isolating compounds from plant. i have got 2 types of crystals,one is needle like and another cubic in shape in a vial. now from here how can i separate them? any technique??

03 April 2015 3,193 7 View

Is a cDNA synthesis kit for realtime PCR compatible with conventional end point RT PCR?

I have been synthesizing cDNA using QuantiTech Rev Transcrp (Qiagen) kit to see the expression of TFF1 gene. In the label of the kit box it says that this kit synthesize cDNA which is specific for...

11 December 2014 9,821 9 View

Can anyone assist with identifying Plasmids from Glycerol stock?

07 August 2014 5,346 13 View

Can anyone help with a PCR optimization problem?

I am doing qPCR for TFF1/pS2 gene expression for the sample using reference compound Estradiol. As such, I am using gradient temperature for optimization. But I don't get any Band for my gene....

05 June 2014 1,038 27 View

Total flavonoid content calculation

I have done TFC. The procedure is as follow: # I dissolved 1 mg extract in 1 ml MeOH. # 100 ul of extract + 100 ul of 2% AlCl3 was mixed in 96 well and incubate for 15 min. # Absorbance was...

03 April 2014 2,003 2 View

How to prepare a primer solution?

I have primers from Integrated DNA Technologies in lyophilized form. The amount of Oligo is 25.5 nMoles = 0.16 mg. Now how should I dissolve it? Which solvent should I use? And how much of it?

02 March 2014 3,016 11 View

Is my RNA concentration too low to synthesis cDNA?

I have RNA , yield concentration 149 ng/ul in 50 ul stock. Now I want to synthesis cDNA using Quantitech Reverse Transcription kit (Qiagen). In the Protocol they suggest maximum 1ug of RNA to use...

01 February 2014 2,726 9 View

Can anyone advise on a cell culture problem?

I am culturing MCF-7 cancer cell line in RPMI with phenol red and without phenol red for my experiment. 10% FBS is used. The passage is 42. For the last couple of passages I see that the cells are...

31 December 2013 8,893 11 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View