I need to know some basic idea about luciferase reporter gene assay, using MCF7 breast cancer cell line.
I have LC-MS spectra of extract where I have the PDF format of the spectra. I sent my extracts to a company who provided me with the pdf only (Not the original file of the software). The library...
02 March 2018 3,307 4 View
I have synthesized cDNA from 1ug of RNA using Quantitech Reverse Transcriptase RT Kit from Qiagen which is for Real Time PCR use. But in out lab we have Conventional RT-PCR. Can i use this cDNA...
07 August 2015 3,770 12 View
I am isolating compounds from plant. i have got 2 types of crystals,one is needle like and another cubic in shape in a vial. now from here how can i separate them? any technique??
03 April 2015 3,355 7 View
I have been synthesizing cDNA using QuantiTech Rev Transcrp (Qiagen) kit to see the expression of TFF1 gene. In the label of the kit box it says that this kit synthesize cDNA which is specific for...
11 December 2014 9,996 9 View
I have three Plasmids in glycerol stock. I labelled them and kept in -80 freezer for 6-7 months. Now when I wanted to take them out from the freezer I found the the labels disappeared. Now is...
07 August 2014 5,499 13 View
I am doing qPCR for TFF1/pS2 gene expression for the sample using reference compound Estradiol. As such, I am using gradient temperature for optimization. But I don't get any Band for my gene....
05 June 2014 1,178 27 View
I have done TFC. The procedure is as follow: # I dissolved 1 mg extract in 1 ml MeOH. # 100 ul of extract + 100 ul of 2% AlCl3 was mixed in 96 well and incubate for 15 min. # Absorbance was...
03 April 2014 2,146 2 View
I have primers from Integrated DNA Technologies in lyophilized form. The amount of Oligo is 25.5 nMoles = 0.16 mg. Now how should I dissolve it? Which solvent should I use? And how much of it?
02 March 2014 3,214 11 View
I have RNA , yield concentration 149 ng/ul in 50 ul stock. Now I want to synthesis cDNA using Quantitech Reverse Transcription kit (Qiagen). In the Protocol they suggest maximum 1ug of RNA to use...
01 February 2014 2,907 9 View
I am culturing MCF-7 cancer cell line in RPMI with phenol red and without phenol red for my experiment. 10% FBS is used. The passage is 42. For the last couple of passages I see that the cells are...
31 December 2013 9,038 11 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View