29 Questions 93 Answers 0 Followers
Questions related from A.K.M. Moyeenul Huq
I have LC-MS spectra of extract where I have the PDF format of the spectra. I sent my extracts to a company who provided me with the pdf only (Not the original file of the software). The library...
03 March 2018 3,249 4 View
I have synthesized cDNA from 1ug of RNA using Quantitech Reverse Transcriptase RT Kit from Qiagen which is for Real Time PCR use. But in out lab we have Conventional RT-PCR. Can i use this cDNA...
08 August 2015 3,721 12 View
I am isolating compounds from plant. i have got 2 types of crystals,one is needle like and another cubic in shape in a vial. now from here how can i separate them? any technique??
04 April 2015 3,310 7 View
I have been synthesizing cDNA using QuantiTech Rev Transcrp (Qiagen) kit to see the expression of TFF1 gene. In the label of the kit box it says that this kit synthesize cDNA which is specific for...
12 December 2014 9,945 9 View
I have three Plasmids in glycerol stock. I labelled them and kept in -80 freezer for 6-7 months. Now when I wanted to take them out from the freezer I found the the labels disappeared. Now is...
08 August 2014 5,436 13 View
I am doing qPCR for TFF1/pS2 gene expression for the sample using reference compound Estradiol. As such, I am using gradient temperature for optimization. But I don't get any Band for my gene....
06 June 2014 1,129 27 View
I have done TFC. The procedure is as follow: # I dissolved 1 mg extract in 1 ml MeOH. # 100 ul of extract + 100 ul of 2% AlCl3 was mixed in 96 well and incubate for 15 min. # Absorbance was...
04 April 2014 2,101 2 View
I have primers from Integrated DNA Technologies in lyophilized form. The amount of Oligo is 25.5 nMoles = 0.16 mg. Now how should I dissolve it? Which solvent should I use? And how much of it?
03 March 2014 3,154 11 View
I have RNA , yield concentration 149 ng/ul in 50 ul stock. Now I want to synthesis cDNA using Quantitech Reverse Transcription kit (Qiagen). In the Protocol they suggest maximum 1ug of RNA to use...
02 February 2014 2,847 9 View
I am culturing MCF-7 cancer cell line in RPMI with phenol red and without phenol red for my experiment. 10% FBS is used. The passage is 42. For the last couple of passages I see that the cells are...
01 January 2014 8,984 11 View
If I don't have DEPC/DEPC treated water, what alternative can I use to make my working pipette, tubes, tips RNase free ? And how?
12 December 2013 8,556 8 View
I want to extract and purify RNA from cell line using Qiagene RNeasy plus mini kit.
10 October 2013 5,476 0 View
I have got competent cell DH5 alpha from a company. Can I grow them for more and use for transfection work in future? If possible then how to grow and extract the cells from growing media and store?
08 August 2013 8,599 3 View
I am going to perform transfection of plasmids. I have got them from Addgene. 1st time I extracted plasmid using a sample mini prep kit from a company the quality was good. But next time I used...
07 July 2013 4,200 2 View
I have received transformed DH5 alpha competent cell from an USA supplier that contain my target DNA. I kept it in 4 refrigerator. Now I want to transfer the culture to another lab which is 1...
06 June 2013 8,817 12 View
What is the best way to seed cells in 24 well plates? Can we seed in the all wells? Why people don not use the outer edge well for cell seeding?
05 May 2013 3,529 8 View
For PCR work and plasmid DNA storage which water is best to use? Can ultra pure water (DNase and RNase free) serve the purpose of nuclease free water? What is the difference?
05 May 2013 8,620 7 View
We have a reverse transcriptase PCR-thermo cycler in our lab. I want to study 4 different gene expressions and have the primers. Can I do it? Can anyone suggest any protocol?
05 May 2013 9,841 5 View
I am doing some cell based assay. I am culturing cells in 25 cm2 cell culture flask. now for my assay I need to seed 1X10^5 cells/well in 24 well cell culture plate. Now, how can I calculate the...
04 April 2013 6,774 5 View
I am working with MCF7 breast cancer cell lines. Now I want to shift my lab. I am culturing the cells and maintaining in cell culture flask. Now how can i transfer this cell lines to another lab....
04 April 2013 6,256 12 View
I want to do PCR for the first time. I am a little confused about one step and two step PCR. Which reagent to use? Is there any difference in choosing reagents for one or two step?
04 April 2013 4,447 12 View
I need to know some basic idea about luciferase reporter gene assay, using MCF7 breast cancer cell line.
02 February 2013 7,966 0 View
I am writing up a manuscript. I want to do proof read before submission to any journal. How can i check plagiarism ? I need an effective plagiarism software.
01 January 2013 5,426 3 View
Is ii possible to run HPLC analytical crude extract of Hexane and DCM using C18 column? How to prepare extract sample and what will be the choice of solvent for mobile phase?
01 January 2013 3,525 12 View
I want to run HPLC for my Water extract. For my plant no paper found to chose a solvent system for HPLC.
12 December 2012 7,565 9 View
We have Waters PDA Prep HPLC in our lab. Suddenly I am facing problem with fluctuation of pressure. What are the factors that can influence the pressure? How to solve this problem?
12 December 2012 4,178 17 View
How acid/base can give better separation of spots? Is it called derivatization? How do they work? Can anyone suggest a book that has a clear concept about it?
11 November 2012 1,294 2 View
Sometimes I see people adjust the pH of the mobile phase in TLC. How does this help in separation?
10 October 2012 8,889 5 View
I am trying to develop a solvent system for HPLC analysis of my extracts.
10 October 2012 1,979 22 View
