Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

Is it possible to full-couple the interFoam solver with the chtMultiRegionFoam solver?

We need to calculate multiphase stream of the one substance in the air atmosphere (this is why we using interFoam), but also we need to get heat transfer data between the substance, solid obstacle...

11 January 2021 872 2 View

Does anyone have experience with Land use/land cover data extraction from Second military survey maps?

I am trying to create polygon shapefile with land use from Second military survey using Arcmap. However, I really have hard time with reading the map, more correctly with boundaries between...

07 November 2020 6,018 3 View

How do I remove Bacillus sp. spores from Kraft Paper machine?

In the pulp and paper company that I work in, samples from paper, water and pulp from the paper machine gave information about existing spores of Presumptive Bacillus cereus. Our paper is used...

03 November 2020 1,726 10 View

What volume of a cell lysis buffer does one use for cells?

I have certain cells that are contaminant cells in a suspension of cells. I found a buffer that selectively removes them, but a lot of protocols don't specify what volume one should use for that...

03 November 2020 6,330 3 View

Best protocol to perform Western Blot from mice brain?

I am trying to look at the expression of c-fos nuclear protein in mice brain using WB technique. Can anyone suggest a protocol to extract the proteins? Better harsh homogenate before sonication?...

27 October 2020 5,272 2 View

Checking purity of sperm suspension?

I have sperm cells extracted from epididymis of a mouse. I would like to check and quantify their purity. My first idea is to use an intercalating dye like Hoechst and then see what fraction of...

25 October 2020 8,600 1 View

What do you think now of virtual teaching compared to face-to-face teaching of finished transmission of knowledge?

21 September 2020 4,178 10 View

How to store W/O droplets for over 10 days using paraffin oil with Span 80?

I am currently using a PDMS chip to produce water droplets with a diameter of 200 um in paraffin oil with 3wt%. After a couple of hours, the stationary droplets stored in the chip starts to form...

18 August 2020 2,459 3 View

How do you use factor analysis on categorical variables?

Hi, I'm trying to identify how similar different individuals are in their body traits. Normally, I would use a PCA or DFA, depending on my question. However, I have only categorical variables...

20 July 2020 4,171 1 View

Is Polaromonas sp. being misidentified by silva as Burkholderiaceae, rather than Comamonadaceae?

I recently ran some 16S sequencing data using the DADA2 pipeline using the silva database (silva_nr_v132_train_set.fa) for taxonomic assignment. Strangely, the genus Polaromonas sp. is being...

31 December 2019 2,401 4 View

Does the current demand of research of GLOFs and Geo-Risk-Managment fit into a Master-Thesis in "Applied Geoscience" ?

In the increasing heating of the atmosphere due to climate change Glacial Lake Outburst Floods (GLOFs) are becoming more critical in the current century. Last decades GLOFs have already taken...

04 May 2019 5,325 3 View

Does water privatization stand to support human life or it evolves to become a threat in the future?

UN says that in just 10 years, every 2 out of 3 people will be living in any country would be struggling to meet demand for water, But Singapore has no aquifers or lakes, assures that its...

03 April 2019 9,433 20 View

Any suggestions for software that will interpolate test hole data in 3D?

I'm looking at Earth Volumetric Studio, Arc Hydro Groundwater with Subsurface Analyst add on, and Hydro GeoAnalyst. I'm working with large testhole data sets and want to create 2D cross sections...

24 February 2019 7,157 3 View

Any suggestions for software that will interpolate test hole data in 3D?

I'm looking at Earth Volumetric Studio, Arc Hydro Groundwater with Subsurface Analyst add on, and Hydro GeoAnalyst. I'm working with large testhole data sets and want to create 2D cross sections...

01 February 2019 473 3 View

Why does there a record temperature fall in the recent years if climate change really cause rise in it?

Isn't temperature fall and down related to geological and astronomical dynamics rather than anthropogenic!

27 December 2018 2,368 5 View

Why does there a record temperature fall in the recent years if climate change really cause rise in it?

Isn't temperature fall and down related to geological and astronomical dynamics rather than anthropogenic!

11 December 2018 9,825 6 View

What is the best method for estimate ciliate population in lakes?

One of the main calculation method for ciliates abundances is using Madoni(1984) sub-sample methodology. However for ciliates is anybody using other methods and how, to calculate abundance in lake?

22 September 2018 7,320 2 View

What is the best method for estimate ciliate population in lakes?

One of the main calculation method for ciliates abundances is using Madoni(1984) sub-sample methodology. However for ciliates is anybody using other methods and how, to calculate abundance in lake?

08 September 2018 1,465 2 View

Great Lakes geochemistry: What's a good venue?

Our research group is working on a rather large pair of manuscripts summarizing geochemistry from 12 sediment cores from all 5 of the Laurentian Great Lakes. MS1 is a literature summary of metals...

04 May 2018 1,915 3 View