Does anybody have a protocol to calculate double-stranded RNA melting point using SYBER staining (eg. SYBER Gold) and qPCR machine?

Xavier Bofill-De Ros @Xavier-Bofill-De-Ros

12 March 2018 0 9K Report

I would like an easy method to measure the thermodynamic differences between two RNA structures after introducing a couple of mutations points.

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