Does anybody have a protocol for RNA extraction from trigeminal ganglion which works well?

Sonali Chaturvedi @Sonali_Chaturvedi

03 March 2018 1 6K Report

Does anybody have a protocol for RNA extraction from trigeminal ganglion from mice which works well?

Mushtak T. S. Al-Ouqaili

Dear Chaturvedi

I worked on RNA extraction from different protocol . I think

TRIzol RNA Isolation Reagents is suitable for you purpose. please see the following link.

https://www.thermofisher.com/us/en/home/brands/product-brand/trizol.html?s_kwcid=AL!3652!10!84043926490924!84043943239109&ef_id=WHCpWQAABYXZCqil:20180322071849:s

Hopeful to be benefit for your dear. Regaed

Badges
Science topic

More Sonali Chaturvedi's questions See All

Is there any literature on induction of acyclovir resistant HSV1 in cell lines?

I am looking for a protocol for induction of acyclovir resistance in HSV1 in epithelial cell line. Please share with me if you have any.

05 June 2018 4,287 1 View

Does anybody have a detailed protocol for HSV1 titer from tear film of mice?

03 April 2018 8,708 0 View

Is there a web interface for 2D classification and building 3D structure from Cryo-EM images?

10 November 2017 3,050 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View