Preferably using Matrigel as the matrix to be invaded. Time of incubation in serum starved condition, time of culture and concentration of Matrigel bed is what I am looking for.
Hello,
I don’t have previous experience with the cells lines you’re working with, you’ll have to test differen conditions in your lab to see which ones fits best for an invasion assay.
I’ve performed invasion assays for rhabdomyosarcoma cells, culturing them until reaching a confluence state of 70 - 80%. Then, overnight incubation with starving medium.
Matrigel concentration it’s difficult to guess. I’ll try different concentrations with your cells until getting significative cell invasion. I usually go with 4 mg/mL for a thin layer, but again, it varies from cell line to cell line.
It’s not a protocol, but I hope it sheds some light to the matter.
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