We are looking for a good protocol to test whether mouse monocytes/macrophages would bind our monoclonal antibody and therefore "eat" or kill the cells for which the antibody is specific. Obviously, we need to perform that test (it exists) in vitro. Any suggestions?
Hi Gilles,
There are a lot of good protocols out there to look at that. You should first decide if you want to work with monocyte-like or macrophage-like phagocytes. There will be a series of differences in the levels and specificity of phagocytosis performed and even on the type of assays you can perform depending on your choice. Do you want to perform assays with cells in suspension or adherent to a plate? Do you want to see the phagocytosis by microscopy, colorimetry, cytometry or more than one method? What's the relative size of the target cells? I think that once you have those questions answered you'll be able to find the protocol you want. As an example of one protocol (but for human cells) you can look at this paper: http://www.ncbi.nlm.nih.gov/pubmed/20520838.
thanx. I would like to test mouse monocytes by cytometry or colorimetric assay. The target cells are human PBMC or a cell line which grow in suspension
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology
- Virology
- Virus