When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

SPSS - Merging files by ID numbers that contain letters?

Hi, I'm trying to merge files from a data base of 20 files, each containing about 4000 subjects. Unfortunately the ID numbers are actually a combination of numbers and letters (e.g., b1458219)....

15 February 2021 5,833 3 View

How would I go about getting an expert review on the interview questions for the dissertation?

Good Evening, My name is David Marshall and I am Doctor of Management Student at Colorado Technical University Online. I just recently received approval from the IRB to start data collection on...

08 February 2021 346 7 View

How can I quantify the color of a solution?

I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...

08 February 2021 2,673 1 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

How does DMSO percentage affect ELISA?

Hello, In my sandwich-type ELISA which is testing for small molecule inhibition of a protein-protein interaction, it seems that DMSO percentage and dilution protocol are important. I...

31 January 2021 6,771 1 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

How to find out or construct(?) the right priors from data of previous experiment to use in a bayesian glm?

I have the following equation, Eating choice (Yes/No)~ Box type (A/B/C). So the question is whether a certain box type affects eating choice in groups of dogs. The eating choice of each member in...

10 February 2021 6,024 9 View

Is it possible estimating environmental conditions with a Bayesian approach?

In ecology specific species can be used to get an indication/estimation of the environmental conditions. A simplistic example, lets assume I find a pike (Esox lucius). We know this species does...

01 February 2021 10,034 4 View

Implementation code for MCMC estimation for CIR stochastic differential equation?

Hello fellow researchers, I am doing a research which involves estimating the parameters of the Cox Ingersoll Ross (CIR) SDE using a Bayesian approach. I propose using the Euler scheme in my...

04 December 2020 8,176 1 View

Implementation code for MCMC estimation of generalized Pareto distribution?

Hello fellow researchers, I am doing a research in extreme value theory where I have to estimate the parameters of a generalized Pareto distribution using a Bayesian approach. I would really...

04 December 2020 4,707 5 View

Beta-binomial distributions using BRMS in R - How should I use the custom_family argument?

I'm trying to compute a beta-binomial model using the R package brms. However, beta-binomial distributions are not native to this package, so I'm trying to use the custom_family argument, to...

10 November 2020 9,265 3 View

How can I generated Simulated/Predicted Effects from an MRQAP model?

I want to generate some nice prediction plots from my MRQAP model. I've laid out my process below, and would be very grateful to get anyone's insight, as I'm not seeing much written about this...

05 November 2020 1,215 2 View

If a researcher, under specified sampling precision, report the Probability of Replication, does that address the abuse and problems with NHST?

08 October 2020 7,014 3 View

How to run and evaluate Bayesian CFA model fit using R ?

Hi, Does anyboby know an R tutorial to run a Bayesian CFA and recommandations concerning the model fit evaluation and adjustment indexes of this type of analysis? I need to run a Bayesian CFA...

06 October 2020 5,819 2 View

Do anyone can introduce a link for downloading "DBNet tool" software?

It is a tool for mapping Dynamic Fault Trees into Dynamic Bayesian Networks.

03 October 2020 2,880 3 View