Does anybody faced problem to clone promoter region in TOPO vector?

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Robert Adolf Brinzer

Was the band you cut out of your gel definitely 2kbp long? have you checked other colonies?

Amy Klocko

How did you initially check your TOPO clones? Keep in mind that if you do PCR with the M13F and R primers those sites are offset from the MCS and will add 200 bp of length to your fragment.

Charli Kaushal

Robert Adolf Brinzer Thak you! yes, I did. Amy Klocko Thank you.