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More than 33 million infections with more than one million deaths worldwide as of 29 Sept 2020, the Wuhan pandemic is a around 10 months long nightmare and showed no signs of improvements. The...

28 September 2020 9,840 6 View

Role of Ivermectin in managing COVID-19 pandemic...?

03 April 2020 4,784 11 View

Use of Large Waveguide Port Extension Coefficients?

I have tried to design a third iterated Sierpinski Gasket fractal antenna of a standard 88.9 mm height for multiband operation. This antenna resonates at 0.52 GHz, 1.752 GHz, 3.712 GHz and...

28 December 2019 6,168 2 View

Like Trees of Delhi which gives easy keys for tree identification in Delhi is there any book of similar kind for Uttarakhand State flora ?

15 April 2017 5,515 8 View

How to solve Partial differential equations by using finite element method?

I am trying to solve set of PDE's by using Gelarkin Finite element method for gaseous phase adsorption of VOCs on activated carbon for drawing breakthrough curves. I have used a quadratic...

03 January 2017 5,777 4 View

Has anyone run dsRNA immunoblots?

Hi everyone I am trying to run an immunoblot for dsRNA molecules of specific lengths. I have been running 5% PAGE gels and transferring the RNA using semi-dry transfer @ less than 10V. Note: the...

22 August 2016 3,101 1 View

Ways around expressing a protein which is seemingly toxic for the cells at higher concentrations in mammalian cancer cell line?

Hi everyone Background: Expressing a membrane protein in A549 cells (lung epithelial cancer cell line) using a expression vector under the constitutive CMV promoter. I am able to detect the...

29 March 2016 1,027 3 View

How to express a fusion protein where one of the protein is a receptor protein?

28 January 2016 6,864 4 View

Disadvantages of using long questionnaire?

20 January 2016 771 8 View

Any suggestion for running generalized estimating equation (GEE) in SPSS?

26 November 2015 5,414 7 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

Does anyone have a sequence for the human CD34 promoter?

I'm looking for the human CD34 promoter sequence to engineer into a vector construct. Can anyone recommend a good source for this sequence? Thanks very much, Laura

25 February 2021 8,205 1 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Why are Sanger Sequencing reads interrupted in some edited clones?

I have a Crispr/Cas9 edited bulk population and I can briefly check the indel ratios in the bulk population using Sanger sequencing. After cloning single cells, I wanted to check the indel types...

18 February 2021 8,198 6 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View