We are constructing a plasmid to express a gene in native Aeromonas spp. isolates. They are resistant to ampicilin and sensitive to tetracycline, so we need to substitute the resistance cassette.
Thanks in advance.
Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.
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Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
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I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
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Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
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I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
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I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
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