Does any one know how I can find sequence of pAdenovator-CMV5?

More Nasrin Haghighi's questions See All

Have you ever had any problem to confirm cloning by restriction analysis?

I have made a construct including 2 PCR produced inserts and a CMV-EGFP-bGH poly A between them. At first, insert 1 was created by PCR and cloned into pSL1180. Cloning was confirmed by...

02 March 2018 8,541 2 View

Does anyone have any kind of experience with viral homologous recombination?

Hi I am using Homologous Recombination to make change in my virus DNA with the plasmid construct which I have already prepared . I have heard this method is really hard and time consuming to be...

02 March 2018 9,960 7 View

Why pET 21 should be reversed for displaying in software such as CLC or Snapgene?

I am trying to insert my fragment in pET21 plasmid, but if I do not reverse pET21 plasmid in CLC or other software, the direction of restriction sites will be disrupted. I was wondering if anyone...

31 December 2017 5,580 4 View

Commercial IL12 expressing plasmid?

I am going to express IL12 in my final construct. As IL12 has two subunits and they need to be linked in order to have expression, I prefer to buy a ready construct to be sure of the expression....

10 November 2017 4,232 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

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05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

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05 August 2024 2,570 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

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28 July 2024 7,559 1 View

What is the best way to drop a plasmid?

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25 July 2024 2,532 3 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View

Asst.Prof.Muthanna H. Hussain

Sorry, I wish I can help you.

Pawel Jaworski

Try to ask mpbio's support for the sequence, but I'm afraid it might not be sequenced, even partially.

https://www.mpbio.com/product.php?pid=11AES2022

Benjamin THOHA Thomas

I m not sure this sequence is available but you can confirm with mpbio