Nasrin Haghighi

5 Questions 5 Answers 0 Followers

Questions related from Nasrin Haghighi

Does anyone have any kind of experience with viral homologous recombination?

Hi I am using Homologous Recombination to make change in my virus DNA with the plasmid construct which I have already prepared . I have heard this method is really hard and time consuming to be...

03 March 2018 9,917 7 View

Have you ever had any problem to confirm cloning by restriction analysis?

I have made a construct including 2 PCR produced inserts and a CMV-EGFP-bGH poly A between them. At first, insert 1 was created by PCR and cloned into pSL1180. Cloning was confirmed by...

03 March 2018 8,516 2 View

Does any one know how I can find sequence of pAdenovator-CMV5?

Could you please introduce me a database to find this plasmid sequence? I could not find it in Addgene and any where else that I know. Thanks

01 January 2018 1,597 3 View

Why pET 21 should be reversed for displaying in software such as CLC or Snapgene?

I am trying to insert my fragment in pET21 plasmid, but if I do not reverse pET21 plasmid in CLC or other software, the direction of restriction sites will be disrupted. I was wondering if anyone...

01 January 2018 5,543 4 View

Commercial IL12 expressing plasmid?

I am going to express IL12 in my final construct. As IL12 has two subunits and they need to be linked in order to have expression, I prefer to buy a ready construct to be sure of the expression....

11 November 2017 4,073 2 View