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Questions related from Nasrin Haghighi
Hi I am using Homologous Recombination to make change in my virus DNA with the plasmid construct which I have already prepared . I have heard this method is really hard and time consuming to be...
03 March 2018 9,929 7 View
I have made a construct including 2 PCR produced inserts and a CMV-EGFP-bGH poly A between them. At first, insert 1 was created by PCR and cloned into pSL1180. Cloning was confirmed by...
03 March 2018 8,525 2 View
Could you please introduce me a database to find this plasmid sequence? I could not find it in Addgene and any where else that I know. Thanks
01 January 2018 1,606 3 View
I am trying to insert my fragment in pET21 plasmid, but if I do not reverse pET21 plasmid in CLC or other software, the direction of restriction sites will be disrupted. I was wondering if anyone...
01 January 2018 5,553 4 View
I am going to express IL12 in my final construct. As IL12 has two subunits and they need to be linked in order to have expression, I prefer to buy a ready construct to be sure of the expression....
11 November 2017 4,084 2 View