Hi Adil, we use the Developmental Studies Hybridoma Bank (Iowa University) mouse monocolonal anti-Nestin Rat-401c (the concentrated one, the other one does not work). It works very well 1:200 on 25 um free floating sections, using 1% DMSO and 1% triton in the blocking (Ab diluted in the blocking), O/N at 4°C. It nicely stains the stem cells in the subventricular zone of the lateral ventricle.

Thanks, Pietro and Shelanah. 

Thanks, Iain.

I ordered your antibody. 

Thanks Frederic for your explanation.

Appreciated, Ill stain my sections and get back to you.

I just have a question,If there any other anti-body that I can use it with nestin to obtain progenitor cells? Or preferable not to use nestin at all for in vivo.   

Thanks Iain,

That helped a lot.

Nestin is a marker for stem cells not for differentiated neurons. We use Mouse anti-Nestin (Chemicon; MAB 353) antibody for labeling stem cells.

Please see our latest publication where we have used Nestin as stem cell marker.

Patro et al., Differential temporal expression of S100b in developing rat brain in Cellular Neuroscience, 2015.

Thanks Patro, I used the Abcam antibody (mouse IgG), product number ab6142, on adult mouse brain sections. I got almost smiler results to the one you presented in your paper with P21 Hippocampus. I was hoping for better expression, so am going to retry it with more fresh mouse brains.

Very nice staining.