I want to know which one is more suitable for dendritic cell transduction. I see that in:
https://www.cell.com/cell/pdfExtended/S0092-8674(15)00829-6
PAX2 plasmid is used for dendritic cells transduction. But psPAX seems to be common plasmid used for LV packaging.
Hello Indumati,
I might be wrong but I think that the authors of the paper you are citing wrote PAX2 instead of psPax2.
In "experimental procedures" they are saying that viruses were produced using psPax2, while in "supplemental experimental procedures" they refer to it as Pax2. Besides I have always used psPax2 for packaging my LVs and when you look for PAX2 on Addgene, the only plasmid that pops out is a lentiviral backbone vector encoding a protein called PAX2, hence not a packaging vector at all.
Hope it helps
Francesco
I am doing a lot of lentivial packing but I never hear from a PAX helper plasmid. Francesco is right, I also think it just was an abbreviation.
Thanks for the response. That explains why I didn't see any transduction events.
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