Does any have any success performing a western blot and probing for Metallothionein?

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Jan Voskuil

You need to run gels fit to resolve low molecular weight proteins to start with. I would recommend NuPage system, probably the Novex4-12% in combination with MES buffer (resolving 2.3kDa-160kDa). Make sure not to run off the front. For your blot you need increased methanol in the transfer buffer for optimal transfer of LMW proteins (15%-20%(v/v).