17 April 2016 1 8K Report

I would like to know if GM130-positive vesicles can be observed in the cytoplasm far from the Golgi complex.

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Abiotic quantification of ROS with DCHF-DA?

Hi together, does anyone have a detailled protocol for the quantification of ROS in an electrolyte solution, i.e. in the absence of any cells? I am currently working on this with the fluorescent...

07 February 2024 8,690 3 View

How can we link these parameters(to stabilize the climate, safeguard ecosystems and improve everyone’s quality of life) to solve environmental issues?

challenging question that I always thinking when I'm trying to think about sustainable environmental conservation.

14 September 2023 537 1 View

APDL-Coding: How to calculat work conducted by external loads?

Hi there, is there an Ansys APDL Command / Method for the work conducted by my external loads? I want to use the work value for further analysis. best regards,

07 August 2023 8,286 1 View

How to complete an incomplete crystal structure in Pymol ?

Dear community, I am currently working on the crystal structure of FOKI. https://doi.org/10.2210/pdb2FOK/pdb Unfortunately, the researches who made the crystal structure havent been able to...

07 July 2023 6,147 1 View

I can't figure it out - EFA or CFA for adapted scales?

Hi everyone, I am currently working on my Master's thesis where I am required to conduct a factor analysis on a scale that was used in a survey. The survey in question was developed by combining...

28 June 2023 5,091 13 View

How to operate Cryojet Remote via RS232 with Arduino/PLC?

Dear all, Can anybody share experience in operating a Cryojet remotely via the RS232 with a Microcontroller? I found no docu nor any HowTo. Thank You! Cheers, Jonas

29 March 2023 6,719 3 View

Big datasets of methane emissions in wet soils?

Hello every I'm looking for datasets looking at methane emissions in wet soils, be it low-lying soils, wetlands, forest swamps, etc.. Any help would be appreciated. Thanks Jonas

09 March 2023 8,102 4 View

Which is the best biotin-azide substrate for click-chemistry to detect nascent protein synthesis with L-homopropargyl-glycine metabolic labelling?

My aim is to detect nascent protein synthesis by click-chemistry of L-homopropargyl-glycine-labelled proteins with biotin azide and detection by western blotting with fluorescently-conjugated...

13 February 2023 8,942 2 View

Should I use TA sites to determine the insertion sites (and counts) for TnSeq experiment using Tn5 transposon?

We generated transposomes following this method Efficient amplification of multiple transposon-flanking sequences. Now I am analyzing the data, however, most of the pipeline available online are...

09 February 2023 3,368 1 View

Is there a method to generate functions automatically in the prediction modelling process ?

Dear researchers, Selecting a function of the right form (linear, polynomial, exponential, power law ...) to fit a set of data usually requires the use of some knowledge and some trial-and-error...

16 January 2023 5,425 0 View

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Senescence-associated beta galactosidase staining is False Positive in control group?

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