Do you know of any article which does a Monte Carlo Simulation assessing measurement invariance in R studio?

Hey! I am a psychology student working on a small project for one of my seminars. The goal is to present to fellow students how different sample sizes affect different ways of assessing...

19 January 2021 4,618 9 View

How can i get my data to be in normal distribution?

The error rate data i have has a strong right skewness and therefore violates the assumption of normal distribution for using an ANOVA. I tried transformation by square root and log10. After...

01 October 2020 8,906 9 View

What is the current state of User Interface Definition Languages?

Hi everybody, in one of our research projects we are currently thinking about generating User Interfaces based on a model representation. I dived into this topic a couple of years ago and still...

15 September 2020 5,350 1 View

Good solar PV Cost country paneldata?

13 August 2020 2,739 4 View

CalculiX Load Beam with moment that follows nodal rotation?

Hello, This is propably a primitiv question but I am trying to figure out how to implement a moment at the tip of a (for simplification) beam around the z axis. It should follow the nodal...

01 April 2020 3,678 3 View

How do AFDyes(TM) compare with Alexa Fluor?

Just wondering due to the stark price difference between AFDye 594 Cadaverine (Click Chemistry Tools) and Alexa Fluor 594 Cadaverine (TFS). From what I read they are supposed to be structurally...

11 March 2020 9,532 2 View

How to prevent silane crosslinkage at pH near to neutral, in resins that are fully waterborne?

Hello, We are working intensively on silane usage as a coupling agent for acrylic polymers. We have a resin with more than 65percent of water and an acrylic monomer, which we polymerize on glass...

14 January 2020 726 1 View

ISO 527 difference of type A and Type B for testing?

I'm planning an experiment with ISO 527. I would like to know what the difference are when testing a type A ( with narrow parallel-sided section and tabs) compared to a type B (rectangular shaped)...

25 February 2019 570 2 View

Increasing solubility of 20-50 nm diameter Carboxylate modified Polystyrene Nanoparticles?

We want to inject these into a living insect. They come in a solution that includes a surfactant that will harm/kill the insect after injection and they appear to aggregate heavily when the...

12 February 2019 9,892 2 View

Is there a ROS scavenger which would not quench carbon centered radical?

Hello, I am looking for a compound which could selectively quench reactive oxygen species while not quenching carbon centered radicals (for a polymerization reaction). Thanks a lot.

11 December 2018 8,494 7 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View