How is the future of hydropower development and engineering heading over the next 5-10 years and why is it important?

Everywhere there is so much going in pumped storage and tidal. In these time we see more innovative use of technology, faster responding machines, more reliable equipment, and more efficient...

04 May 2018 268 0 View

How can prove the impacts of climate change on road infrastructure?

I need your helps dear coleagues.

31 December 2017 2,056 4 View

How does climate change affect flora and fauna?

Dear collegues, experts etc., Before 4 years ago I have asked: How does climate change affect flora and fauna"? Thank you very much for all positive answers given to us! Now I am asking: How do...

11 December 2017 7,637 16 View

Why celebrate the new year?

Before all I've wishes:Dear our colleague,Thanks a lot for your congratulation, suggestions, comments etc, give to us throught the very nice message!Dear our colleague,hour after hour of...

11 December 2016 2,446 12 View

What are the reasons who I, you, he/she, we, you and they live/s?

04 May 2016 8,285 4 View

What is the index of expected longevity and how is this index to the local ommunity/indigenous people, where you live/work?

04 May 2016 8,949 3 View

What is the index of education and how is this index to the local community/indigenous people, where you live/work?

Dear colleagues, I have my greetings to you from our country, Albania!I know lot of things in theory, but I am practical person, so I am interested to know something about the index of education...

04 May 2016 2,835 3 View

What are respect and psychological violence, report between them?

03 April 2016 9,170 1 View

Would you like take time ... ?

03 April 2016 7,576 2 View

... in these times not normal, happy to have a party?

... in these times not normal, happy to have a party? Yes! Dear colleagues, I wish you happy for many years the Catholic Easter!

02 March 2016 9,107 5 View

No title

02 March 2021 324 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the value of Conference Review Paper?

How much value does a review paper carry in a higher study aspirant student's profile which is presented in a Peer Reviewed Scopus Indexed conference?

27 February 2021 2,068 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View