Do you think of implementing PCR-RT / ELISA for Zika for better detection?

Luis Del Carpio-Orantes @Luis_Del_Carpio-Orantes

06 June 2017 0 10K Report

Because in the past wave of zika on America, it was not always possible to detect and corroborate the cases by RT-PCR, we opted to practice the initial PCR test during the first 7 days, and then a new serology based on IgM / IgG

Badges
Science method

More Luis Del Carpio-Orantes's questions See All

Long COVID treatment?

With the advent of long covid, there are serious doubts about the way to approach diagnosis and treatment, in your experience with which drugs or therapies have treated this condition?

14 May 2023 4,240 0 View

What do you think of the Qeios platform?

It is a platform where you can upload work and receive reviews and feedback, being able to improve the work, in addition to being published in PDF immediately

26 January 2023 6,416 2 View

New treatments about COVID-19?

Do you have used drugs like Baricitinib, tocilizumab or polimerized type I collagen for the treatment of COVID-19?

15 April 2021 7,736 5 View

Do I need a particular consideration to extract DNA from herbarium dried samples that were first preserved with ethanol?

I need to recover DNA from past plant populations of an specific genera. There are a few samples in local herbaria, but some of them were just dried and another were treated with ethanol before...

14 April 2018 7,503 3 View

It would be good to integrate a study group on the diseases favored by arboviruses?

It would be good to integrate a study group on the diseases favored by arboviruses, such as guillain barre and congenital syndrome due to zika or encephalopathy and chikungunya arthritis, among...

13 December 2017 4,725 0 View

Drugs such as sofosbuvir and azithromycin, with anti-zika potential and safety in pregnancy, can reduce the congenital syndrome by zika?

Sofosbuvir and other anti-infective agents such as azithromycin, which may be administered during gestation, have shown efficacy in the treatment of zika, could be implemented in regions of high...

05 August 2017 2,155 1 View

Why during the zika wave, there is an increase in the incidence of guillain barré syndrome, but the direct etiological evidence is extremely low?

In Mexico until June 2017, 1,011 cases of Guillain Barré syndrome have been documented, but only 15 Zika-positive cases have been reported by RT-PCR, which is due to this poor correlation?

22 July 2017 9,574 0 View

What will be the predisposing factor for the direct person-to-person transmission of zika virus?

So far, 13 cases of direct person-to-person transmission of zika virus have been documented, and the only predisposing factor appears to be a high viral load of patients who are critically ill.

22 July 2017 9,146 3 View

What do you recommended to found the etiology of all cases of guillain barre syndrome related to zika

PCR-RT, ELISA or both in blood, urine or other like cephaloraquid liquid, seminal or saliva

01 January 1970 4,290 2 View

Podria existir un nuevo linaje americano respecto del virus Zika, recordando los linajes africano y el derivado asiático??

Las primeras cepas del linaje africano se asentaron en Nigeria y Senegal, la cual evolucionando hasta llegar a Asia, a traves de Malaysia y las islas Yap, que ya forman parte del linaje asiático,...

01 January 1970 4,318 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View