Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Pls inform me if the problem here is in the used red safe. im using the red safe of vivantis co, Malysia. using 5 microliter per 100 ml of agarose?

Why the ladder is always with so closle or attached bands after the 500 bp band whatever i use a type of ladder ( 5types) and also the PCR product is bended. is this has a relation with the used...

05 January 2021 5,211 5 View

Is it important/advisable to write a summary for each theme (and subthemes) in Thematic Analysis?

I am conducting semi-structured interviews with graduate students to explore their academic writing challenges. I am using thematic analysis to analyze the Qual findings. Should I include a...

31 December 2020 3,593 22 View

Using Matlab and the analytical solution for Room acoustic modeshapes, is it possible to plot modes in 3D?

mode shapes similar to attached image.

27 November 2020 4,262 7 View

What tool can I use for creating a Quantative Trait Loci map from SSR data?

I have SSR data for some individual of three hybride How I construct QTL mapping of yield according to these data ?

02 October 2020 502 5 View

Labs for purification, identification & isolation of unknown proteins form native sample of honey and to test versus GS-9L cells in vitro?

I asked for protein purification, identification and isolation of unknown proteins and test versus GS-9L other than that we already got in our patent WO/2014/040605 - form native sample of honey?!

23 September 2020 1,839 3 View

Hi everyone, do you know any GPS logger that we can use to conduct travel time surveys and which is easy to extract the data from ?

We are currently planning to conduct Freight Master Plan in the state of Qatar, one of the requirements is to conduct travel time surveys, therefore we are currently searching for GPS devices that...

22 July 2020 1,889 1 View

How can I quantitatively analyze a sample with carbohydrate content?

Can anyone -who may have hands-on experience in analytical chemistry or photochemistry- help me how can I quantitatively analyze a sample with carbohydrate content?

09 July 2020 3,663 2 View

Any Predictive Markers for Severe COVID-19 in children?

Predictive Markers for Severe COVID-19 Identified

15 June 2020 1,375 5 View

How to critize (Evidence based medicine) this paper?

24 May 2020 5,489 3 View

How can we optimize a water distribution system if we do not have the zones' topology (pipes graph )?

Hello everyone, My issue is about a water distribution system that I am working on a zone of the system where does not exist any plan for the place of pipes. However, we have the place of...

14 September 2020 5,978 6 View

CFD- how to model rigid body dynamics in a flow-driven valve?

Hello, I'm trying to simulate the flow in a pipe containing a bi-leaflet valve whose movement (the rotation angle) is driven by the flow at the inlet using Ansys CFX. I'm using an immersed solid...

23 March 2020 4,609 2 View

Does a bicuspid valve confer any advantage over a tricuspid valve in terms of fluid dynamics in a human heart?

I've been grappling with this question for a very long time. There must be a logical reason to explain why almost all humans have one bicuspid mitral valve and 3 tricuspid valves in their hearts....

01 February 2020 2,644 1 View

Which Oil/Liquid do you use for Schlenk Pressure Relief Valve?

Dear community, I want to equip my Schlenk Line with a Pressure Relief Valve, usually filled with some oil or other viscous liquid to produce bubbles when overpressure of Argon is present. Which...

29 January 2020 1,174 2 View

Custodiol HTK and Terminal warm blood (Hotshot) any thoughts?

We use Custodiol HTKs for our MIMVS (minimally invasive mitral valve surgery program). In some cases before the x-clamp removal we give 400ml of warm blood but there isn't a SOP for such. In your...

02 March 2019 4,280 7 View

How to decide connection SRV (Flanged or Butt Weld) ?

I try to design Relief Valve for my job. Pressure 50 bar, Temperature 490 degC My assumsion is Flanged because its less than 4500# (ASME B16.34 i think). But for High Pressure and High...

10 November 2018 6,874 0 View

Does anyone have an excel/SAS/STATA/SPSS file to calculate STS score for isolated aortic valve replacement?

Also, if you have recently calculated coefficients or weights for the variables, I can create an algorithm.

09 October 2018 5,365 0 View

Simulating blood flow in bioprosthetic heart valve?

Hi everyone Can we consider the flow of blood in the aortic valve of the type low Reynolds turbulence? Best regards

05 June 2018 2,734 3 View

What treatment shoud we choice for moderate functional Mitral regurgitation?? How much time should we wait and watch our patients until a decision?

We know that if we watch our patients with moderate mitral regurgitation we are going to have structural changes in miocardial anatomy those will difficult the future surgical treatment. So why we...

03 April 2018 3,399 4 View

What would be the possible problems that we face with the Alicat-precision gas Mass flow controller using for the Pulsed laser deposition?

10 November 2017 9,318 3 View